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| Strain Number: | 01XN3 | ||
| Common Strain Name: | hGFAP-Cre | ||
| Strain Nomenclature: | C57BL/6-Tg(GFAP-cre)8Gtm | ||
| Release Category (Required for MTA form): C,D | |||
| Animal Health Report in PDF Format | |||
| Strain Description: | Transgenic mouse expressing Cre recombinase under control of the astrocyte-specific human GFAP promoter (Bajenaru et al., 2002). The transgene construct included an internal ribosome entry site (IRES) to allow for co-expression of Cre and beta-galactosidase (LacZ) from the same transcript. Astrocyte-specific expression of Cre and LacZ was confirmed by immunohistochemistry. Beta-galactosidase activity was first detected in the subventricular zone by embryonic day 14.5. GFAP-Cre mice are phenotypically normal. This strain has been used for astrocyte-specific inactivation of several cancer-related genes, including Nf1 (Bajenaru et al., 2002, 2003), Tsc1 (Uhlmann et al., 2002), and Pten (Fraser et al., 2004). | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | Cre recombinase | ||
| Transgene Symbol: | Cre | ||
| Promoter Name: | GFAP, human | ||
| Promoter Symbol: | GFAP | ||
| Current Genetic Background: | C57BL/6 | ||
| Approx. Generation: | 7 | ||
| Organ Site : | Brain | ||
| Husbandry: | Breeder pairs are supplied as a hemizygote (male or female) and wildtype (+/+) C57BL/6 mate. Average litter size is 10. | ||
| Genotyping Information: | Protocol 1: Allele: Tg(GFAP-cre)8Gtm | ||
| Donating Investigator: | Dr. David H. Gutmann | ||
| Key Reference: | Bajenaru ML, Zhu Y, Hedrick NM, Donahoe J, Parada LF, Gutmann DH. Astrocyte-specific inactivation of the neurofibromatosis 1 Gene (Nf1) is insufficient for astrocytoma formation. Mol. Cell. Biol. 22, 5100-5113, 2002. PMID: 12077339 [PubMED Abstract] | ||
| Related References: | Bajenaru ML, Hernandez MR, Perry A, Zhu Y, Parada LF, Garbow JR, Gutmann DH. Optic nerve glioma in mice requires astrocyte Nf1 gene inactivation and Nf1 brain heterozygosity. Cancer Res. 63, 8573-8577, 2003. PMID: 14695164 [PubMED Abstract] | ||
| Bajenaru ML, Garbow JR, Perry A, Hernandez MR, Gutmann DH. Natural history of neurofibromatosis 1-associated optic nerve glioma in mice. Ann Neurol. 57, 119-127, 2005. PMID: 15622533 [PubMED Abstract] | |||
| Fraser MM, Zhu X, Kwon CH, Uhlmann EJ, Gutmann DH, Baker SJ. Pten loss causes hypertrophy and increased proliferation of astrocytes in vivo. Cancer Res. 64, 7773-7779, 2004. PMID: 15520182 [PubMED Abstract] | |||
| Sponsoring Institute: | The National Institutes of Health, NIH NS36996 | ||
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