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| Strain Number: | 01XJ3 | ||
| Common Strain Name: | HB-PLAP(A to G) | ||
| Strain Nomenclature: | FVB-Tg(ACTB-ALPP)03Jrst | ||
| Release Category (Required for MTA form): C | |||
| Animal Health Report in PDF Format | |||
| Strain Description: | Mutant plap gene with A to G transition mutation in a spice acceptor site resulting in a non-functional plap gene product. The transgenic mouse reports G to A transition mutations by reverting the plap transgene to wildtype. Histological assay of heat stable alkaline phosphatase activity detects G to A transition mutations in all tissues of the animal. Useful for detecting mutation distribution resulting from genetic defects and environmental exposure to mutagens. | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | human placental alkaline phosphatase | ||
| Transgene Symbol: | PLAP | ||
| Promoter Name: | human beta actin | ||
| Promoter Symbol: | ACTB | ||
| Current Genetic Background: | FVB/N | ||
| Approx. Generation: | |||
| Organ Site : | |||
| Cryopreservation Mating Scheme: | FVB/N females x homozygous males | ||
| Genotyping Information: | Protocol 1: Allele: Tg(ACTB-ALPP)03Jrst Protocol 2: Allele: Tg(ACTB-ALPP)03Jrst | ||
| Donating Investigator: | Dr. James Stringer | ||
| Key Reference: | DePrimo SE, Cao J, Hersh MN, and Stringer JR. 1998 Use of human placental alkaline phosphatase transgenes to detect somatic mutation in mice in situ. Methods 16: 49-61. PMID: 9774516 [PubMED Abstract] | ||
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