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| Strain Number: | 01XH7 | ||
| Common Strain Name: | HB-Plap(G11) | ||
| Strain Nomenclature: | FVB-Tg(ACTB-ALPP)02Jrst | ||
| Release Category (Required for MTA form): C | |||
| Animal Health Report in PDF Format | |||
| Strain Description: | Mutant human PLAP (ALPP) gene with 7Gs inserted downstream of a 4 G run resulting in a frameshift that yields a non-functional PLAP gene product. The transgenic mouse reports frameshift mutations by reverting the PLAP transgene to wildtype. Histological assay of heat stable alkaline phosphatase activity detects frameshift mutations in all tissues of the animal. Useful for detecting mutation distribution resulting from genetic defects and environmental exposure to mutagens. | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | human placental alkaline phosphatase | ||
| Transgene Symbol: | PLAP | ||
| Promoter Name: | human beta actin | ||
| Promoter Symbol: | ACTB | ||
| Current Genetic Background: | FVB/N | ||
| Approx. Generation: | 5 | ||
| Organ Site : | |||
| Cryopreservation Mating Scheme: | FVB/N females x hemizygous males | ||
| Genotyping Information: | Protocol 1: Allele: Tg(ACTB-ALPP)02Jrst Protocol 2: Allele: Tg(ACTB-ALPP)02Jrst | ||
| Donating Investigator: | Dr. James Stringer | ||
| Key Reference: | Hersh MN, Stambrook PJ, Stringer JR 2002 Visualization of mosaicism in tissues of normal and mismatch-repair- deficient mice carrying a microsatellite-containing transgene. Mutat Res 505:51-62. PMID: 12175905 [PubMED Abstract] | ||
| Related References: | DePrimo SE, Cao J, Hersh MN, and Stringer JR. 1998 Use of human placental alkaline phosphatase transgenes to detect somatic mutation in mice in situ. Methods 16: 49-61. PMID: 9774516 [PubMED Abstract] | ||
| Cao J, DePrimo SE, Hersh MN, Stringer JR. In situ detection of frameshift mutations in mouse cells. Mutat Res. 1998 Nov 3;421(2):163-78. PMID: 9852990 [PubMED Abstract] | |||
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