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| Strain Number: | 01XE1 | ||
| Common Strain Name: | TetO-Cre | ||
| Strain Nomenclature: | FVB/N-Tg(TetO-Cre)3Jig | ||
| Release Category (Required for MTA form): B,D | |||
| Strain Description: | Doxycycline-induced knockouts of a floxed allele can be engineered in mice containing Fabpl4X at -132/rtTA and TetO-Cre. Analysis of recombination using the floxed R26R Cre reporter indicates that the principal site of recombination is in the proximal two thirds of the small intestine with lesser degrees of recombination distally. Recombination occurs in long-lived multipotent stem cells and all descendant lineages. Note that recombination is mosaic: some crypts support recombination while others do not (see Wong et al., PNAS 97:12601 (2000) for illustration. There is no expression in the urothelium or in other extraintestinal sites except the liver: a subset of tri-transgenic mice hemizygous for Fabpl4X at -132rtTA, tetO-Cre and R26R exhibit recombination of R26R in scattered groups of hepatocytes after doxycycline treatment. No recombination is observed in any organ surveyed in the absence of doxycycline. | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | Cre recombinase | ||
| Transgene Symbol: | Cre | ||
| Promoter Name: | Tet operon | ||
| Promoter Symbol: | hCMV | ||
| Current Genetic Background: | FVB/N | ||
| Approx. Generation: | 19 | ||
| Organ Site : | |||
| Cryopreservation Mating Scheme: | FVB/N females x hemizygous males | ||
| Genotyping Information: | Protocol 1: Allele: Tg(TetO-Cre)3Jig | ||
| Donating Investigator: | Dr. Jeffrey Gordon | ||
| Key Reference: | Wong, MH, Gordon, JI 2000. Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection. Proc. Natl. Acad. Sci. USA 97:12601-06 PMID: 11050178 [PubMED Abstract] | ||
| Related References: | Saam JR, Gordon JI. 1999. Inducible gene knockouts in the small intestinal and colonic epithelium. J. Biol Chem. 274:38071-82. PMID: 10608876 [PubMED Abstract] | ||
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