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Strain
Number: |
01XBE |
Common
Strain Name: |
C57BL/6 DNTGFRII |
Strain
Nomenclature: |
C57BL/6-Tg(CD2-Tgfbr2)1Grs |
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Release
Category (Required for MTA form):
B
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Animal Health Report in PDF Format |
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Strain
Description: |
These transgenic mice express a dominant-negative form of the TGF beta type II receptor (DNRII) under control of the human CD2 promoter/enhancer. Both CD4+ and CD8+ T-cells expressed comparable amounts of the transgene, whereas no transgene mRNA was detected in T cell-depleted lymphocyte populations (Lucas et al., 2000). DNRII expression resulted in clonal expansion and progressive hyperproliferation of CD8+ memory T-cells, resulting in dramatically increased leukocyte counts by 30 weeks of age. Homozygous mice exhibited symptoms of decreased activity, loss of weight, and difficulty breathing 4 months before hemizygous animals became symptomatic (Lucas et al., 2004). The disease progressed to leukemia/lymphoma as determined by histology, T-cell receptor monoclonality, and host transferability. |
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Mutation
Information |
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Mutation
Type: |
Transgenic |
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Gene
Name: |
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Gene
Symbol: |
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Transgene
Name: |
transforming growth factor, beta receptor II
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Transgene
Symbol: |
Tgfbr2
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Promoter
Name: |
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Promoter
Symbol: |
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Current
Genetic Background: |
C57BL/6 |
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Approx.
Generation: |
5 |
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Organ
Site : |
hematopoietic |
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Cryopreservation Mating Scheme: |
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Genotyping Information: |
Protocol 1: Allele: Tg(CD2-Tgfbr2)1Grs
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Donating
Investigator: |
Philip Lucas |
Key
Reference: |
Lucas PJ, Kim S-J, Melby SJ, Gress RE. Disruption of T-cell homeostasis in mice expressing a T cell-specific dominant negative transforming growth factor beta II receptor. J Exp Med 191, 1187-1196, 2000. PMID: 10748236.
[PubMED
Abstract]
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Related
References: |
Lucas PJ, McNeil N, Hilgenfeld E, Choudhury B, Kim S-J, Eckhaus MA, Ried T, Gress RE. Transforming growth factor beta pathway serves as a primary tumor suppressor in CD8+ T-cell tumorigenesis. Cancer Res 64, 6524-6529, 2004. PMID: 15374963. [PubMED
Abstract] |
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Sponsoring Institute: |
The National Institutes of Health, National Cancer Institute, ACS |
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