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Strain
Number: |
01XB4 |
Common
Strain Name: |
TetO-HRAS |
Strain
Nomenclature: |
FVB-Tg(tetO-HRAS)65Lc |
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Release
Category (Required for MTA form):
B,D
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Animal Health Report in PDF Format |
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Strain
Description: |
This strain carries an activated HRAS human gene (G12V) under the control of a minimal promoter containing multimerized tet-operons (TetO). TetO will drive expression of the HRAS transgene in the presence of a tetracycline transcriptional activator(tTA), or a tetracycline reverse transcriptional activator (rtTA), in the absence or presence of doxycycline (Dox), respectively. In normal use this strain would be mated with a transgenic strain carrying tTA or rtTA under the control of a tissue specific promoter/enhancer. |
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Mutation
Information |
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Mutation
Type: |
Transgenic |
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Gene
Name: |
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Gene
Symbol: |
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Transgene
Name: |
RAS oncogene
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Transgene
Symbol: |
HRAS
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Promoter
Name: |
Tet Operon |
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Promoter
Symbol: |
TetO |
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Current
Genetic Background: |
FVB/N |
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Approx.
Generation: |
11 |
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Organ
Site : |
All |
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Cryopreservation Mating Scheme: |
FVB/N females x hemizygous males |
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Genotyping Information: |
Protocol 1: Allele: Tg(TetO-HRAS)65Lc
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Donating
Investigator: |
Dr. Lynda Chin |
Key
Reference: |
Chin L, Tam A, Pomerantz J, Wong M, Holash J, Bardeesy N, Shen Q et.al. 1999. Essential role for oncogenic Ras in Tumour maintenance. Nature 400:468-72.
PMID: 10440378
[PubMED
Abstract]
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