Alexander Lazarev
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Speaker: Alexander Lazarev, Pressure Biosciences, Inc., Woburn, MA 01801
Topic: Life Under Pressure: Applications of High Hydrostatic Pressure in Life Sciences
Place: Building 549, Conference Room B, NCI at Frederick, Frederick, MD
Time: Tuesday, June 10, 2008, at 2:00 PM
Abstract: Systems biology studies are gaining momentum driven by success in genomics, transcription profiling, proteomics, and rapidly emerging metabolomic technologies. While powerful and sensitive methods are available for the analysis of nucleic acids, proteins and small molecules, major bottlenecks arise from the limitations of current sample preparation techniques. This seminar will focus on a novel detergent-free sample preparation approach which allows concurrent isolation and fractionation of proteins, DNA, RNA and lipids from cells and tissues. This method relies on a synergistic combination of physical treatment by alternating hydrostatic pressure (Pressure Cycling Technology, or PCT) and optimized reagents resulting in cell disruption, dissolution and partitioning of distinct classes of molecules into separate fractions. Examples of protein, lipid, DNA and RNA extracts from several cultured cells and tissue types will be presented.
The slides to this seminar are in a 4.6 Megabyte PDF file, which can be opened and read by using the free Adobe Acrobat Reader®.
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