Kelly A. Conrads Ciphergen Biosystems, Inc. Office: 301-580-5337 FAX: 301-668-7950 E-mail: kconrads@ciphergen.com Job Title: Field Scientist, Mid-Atlantic Region Ph.D. in Biochemistry from Ohio State University

Speaker: Kelly A. Conrads, Ciphergen Biosystems, Inc., Frederick, MD

Topic: Protein Chip® Technology: A New Tool for Proteome Analysis and Biomarker Discovery

Place: Building 549, Auditorium, NCI at Frederick, Frederick, MD

Time: Tuesday, August 17, 2004, at 2:00 PM

Abstract: Ciphergen's ProteinChip® Technology utilizes Surface-Enhanced Laser Desorption/Ionization (SELDI) which enables the capture, detections and analysis of proteins directly from crude biological samples. The ProteinChip arrays have classical chromatographic surfaces such as normal phase, reverse phase, ion exchange and immobilized metal ion. In addition, ProteinChip arrays with pre-activated surfaces allow covalent immobilization of molecules, such as antibodies, proteins and DNA, for specific affinity capture. ProteinChip Arrays can be prepared and analyzed in a matter of minutes, making it feasible to screen hundreds of samples in a few days to identify proteins whose expression patterns are changing in response to disease or treatment status. ProteinChip Technology has been applied to detect potential biomarkers for numerous diseases including ovarian cancer. Using Ciphergen's Expression Difference Mapping, four potential biomarkers for ovarian cancer were discovered, purified and identified. Next, Interaction Discover Mapping using pre-activated surfaces and antibodies was used to discover additional potential markers by mining deeper into the proteome. The ovarian cancer study is one example of how a comprehensive study can be achieved in a high throughput fashion using Ciphergen's technology.

The slides to this seminar are in a 3.3 Megabyte PDF file, which can be opened and read by using the free Adobe Acrobat Reader®.