MS-Fit Instructions

A peptide-mass fingerprinting tool from the that tries to fit a user's mass spectrometry data to a protein sequence in an existing database and thus suggest the identity of the user's protein. The MS input data should be generated by analyzing the peptides produced by the enzymatic digestion of a user's protein.
MS-Seq MS-Edman
MS-Digest MS-Product MS-Comp DB-Stat MS-Isotope
Database: Instrument:
DNA Frame translation:
Species:
MW of Protein: (from Da to Da) All
Protein pI: (from to ) All
Digest:
      Max. # of missed cleavages:
Cysteines modified by:
N terminus: C terminus:
Sample ID (comment):
Max. Reported Hits:
Possible
Modifications
Mode (default)
User Defined Modification 1:
OR
Homology Mode (select any mode but identity)
Search mode:
Min. # matches with NO AA substitutions:
Peptide Mass shift: Da
Peptide masses are:
Min. # peptides required to match:
Report MOWSE Scores: Pfactor:
Peptide Masses
mass tolerance:
Mass (m/z) Charge (z)
Other peptide-mass fingerprinting servers:
Prediction of potential protein post-translational modifications and single amino acid substitutions: