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-Sample Preparation
-DNA Probe Preparation
-FISH
-CGH
-SKY
-MicroArrays Expression & CGH
-Immunocytochemistry/ Molecular Biology
-Miscellaneous
Sample Preparation
PDF High Resolution Chromosomes
PDF Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique
PDF Metaphase Preparation from Adherent Cells
PDF Metaphase Preparation from Mouse Embryonic Fibroblast (MEF)
PDF Metaphase Preparation from Mouse/Rat Spleen
PDF Preparation of Human Metaphase Chromosomes
PDF Preparation of Paraffin Sections and Frozen Tissue for FISH
PDF Prometaphase Chromosome Preparation from Mouse Spleen (C57Bl/6)
PDF Storage of Chromosome Preparations
PDF Triple Enzyme Mouse Tumor Digestion
DNA Probe Preparation
PDF cDNA Synthesis for RT-PCR Protocol
PDF DNA Preparation from Adherent Cells
PDF DNA Preparation from Blood
PDF DNA Preparation from Cell Lines, High Salt Method
PDF DNA Preparation from Fresh/Frozen Tissue
PDF DNA Preparation from Paraffin Tissue
PDF Isolation of BAC DNA using Autogen 850
PDF Preparation of Alpha-satellite DNA by PCR
PDF Primary Amplification of Genomic DNA using DOP-PCR
FISH - Fluorescence In Situ Hybridization
PDF Antifade
PDF DNA Precipitation and Hybridization (FISH)
PDF FISH Detection
PDF Nick Translation (FISH)
PDF SKY/FISH of Previously G-banded Slides
PDF Slide Pretreatment
PDF Tyramide Signal Amplification (TSA) Detection
CGH - Comparative Genomic Hybridization
PDF Antifade
PDF CGH Detection
PDF DNA Precipitation and Hybridization (CGH)
PDF Nick Translation (CGH)
PDF Slide Pretreatment
SKY - Spectral Karyotyping
PDF Amplification of Flow Sort DNA using DOP-PRC (SKY)
PDF Antifade
PDF DOP-PCR Labeling (SKY)
PDF DOP-PCR Secondary (SKY)
PDF Hybridization (SKY)
PDF PDF DOP-PCR Labeling (SKY)
PDF Precipitation of SKY Kits and QC
PDF Primary Amplification Genomic DNA using DOP-PCR
PDF SKY Detection
PDF SKY/FISH of Previously G-banded Slides
PDF Slide Pretreatment
MicroArrays - Expression & CGH
PDF cDNA Microarray Indirect Labelling
PDF RNA Extraction from Cell Lines or Frozen Tissue
Immunocytochemistry/Molecular Biology
PDF Immunocytochemistry
PDF Immunocytochemistry Followed by Electron Microscopy
PDF Immunocytochemistry Followed by FISH (Version 1)
PDF Immunocytochemistry Followed by FISH (Version 2)
PDF Immunocytochemistry Followed by FISH (Version 3)
PDF Preparation of Protein Extracts for 2D Gel-analysis
PDF RT_PCR Reaction
PDF Western Blot Analysis: Detection of Proteins using Alkaline Phosphatase
PDF Western Blot Analysis: Detection of Proteins using Chemiluminescence
PDF Western Blot Analysis: Preparation of Protein Extracts
PDF Western Blot Analysis: Protein Quantification
Miscellaneous
PDF Collagen Coating Flasks
PDF Giemsa-trypsin Banding

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