National Toxicology Program

CASRN: 100-40-3

Formula: C8 H12

Synonyms/Common Names:

• 4-ETHENYLCYCLOHEXENE (9CI)

Known Uses:

MANUFACTURE OF POLYMERS AND EPOXY RESINS

Short-Term Toxicity

• 14-Day (Gavage)  (C54999) Completed 
  • NTIS No. PB87-116182  (Peer Review 03/29/1985)
    Citation: Toxicology and Carcinogenesis Studies of 4-Vinylcyclohexene (CAS No. 100-40-3) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Gavage)  (C54999) Completed 
  • NTIS No. PB87-116182  (Peer Review 03/29/1985)
    Citation: Toxicology and Carcinogenesis Studies of 4-Vinylcyclohexene (CAS No. 100-40-3) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,50,800, M: 0,75,1200 MG/KG/10 PER GROUP

Long-Term Carcinogenicity

• 2-Year (Gavage)  (C54999) Report Complete 
  • TR-303 (NTIS No. PB87-116182)  (Peer Review 03/29/1985)
    Toxicology and Carcinogenesis Studies of 4-Vinylcyclohexene (CAS No. 100-40-3) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Inadequate Study 
  • Female Rats Inadequate Study 
  • Male Mice Inadequate Study 
  • Female Mice Clear Evidence 
  • Dose: 0,200,400 MG/KG/50 PER GROUP

Special Studies

• Absorption Disposition Metabolism Elimination (Gavage)  (S0084) Completed 
  • Citation: SMITH, B.J., MATTISON, D.R. & SIPES, I.G.: THE ROLE OF EPOXIDATION IN 4-VINYLCYCLOHEXENE-INDUCED OVARIAN TOXICITY, TOXICOLOGY & APPLIED PHARMACOLOGY, 105:372-381,1990 ***** SMITH, ET.AL., COMPARISON OF THE DISPOSITION & INVITRO METABOLISM OF 4-VINYLCYCLOHEXENE IN THE FEMALE MOUSE & RAT, TOXICOLOGY & APPLIED PHARMACOLOGY, 105:364-371,1990
  • M Rats: F344/N; M Mice: B6C3F1
  • Dose: M: 5 MG/ML/MOUSE R: 50 MG/ML/RAT
• Metabolism (Intraperitoneal Injection)  (S0800) Completed 
  • Citation: OVARIAN TOXICITY OF 4-VINYLCYCLOHEXENE AND RELATED OLEFINS IN B6C3F1 MICE: ROLE OF DIEPOXIDES. CHEM. RES. TOXICOL. 1995, 8, 963-969. J.K. DOERR ET AL. OVARIAN TOXICITY AND METABOLISM OF 4-VINYLCYCLOHEXENE AND ANALOGUES IN B6C3F1 MICE. STRUCTURE-ACTIVITY STUDY OF 4-VINYLCYCLOHEXENE AND ANALOGUES. DOERR & SIPES. BIOLOGICAL REACTIVE INTERMEDIATES V. CHAPTER 46. PLENUM PRESS, NEW YORK, 1996.
  • F Mice: B6C3F1
  • Dose: Structure-Activity Studies: at either a dose equimolar to that of VCH (7.5 mmol/kg), or at a lower dose which had been determined fromprevious studies as the maximum tolerated dose by mice over 30 day period w/o significant weight loss. In Vivo Metabolism Studies: The mice were administered a single dose (7.5 mmol/kg), ip, of test com-pound in sesame seed oil.
• Metabolism (Intraperitoneal Injection)  (S0801) Completed 
  • Citation: INDUCTION OF CYTOCHROME P-450 ENZYMES AFTER REPEATED EXPOSURE TO 4- VINYLCYCLOHEXENE IN B6C3F1 MICE. DRUG METABOLISM AND DISPOSITION, VOL. 27, NO. 2, 281-287. J.K. DOERR-STEVENS ET AL., 1998.
  • F Mice: B6C3F1
  • Dose: VCH (7.5 mmol/ky/day x 10 days i.p.), sesame seed oil (vehicle control, 2.5 ml/kg/day x 10 days i.p.), PB (80 mg/kg/day x 5 days i.p.), DEX (100 mg/kg/day x 3 days i.p.), or acetone (ACE) [in drinking water 1 % (v/v) x 5 days]. PB was made up as 3.2% solution (w/v) in 0.9% NaCl, and DEX was in a 2% Tween 80 solution (v/v).

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (169960) Completed 
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Negative 
• Mouse Lymphoma  (971117) Completed 
  •  Positive 
• Salmonella  (609542) Completed 
  •  Negative 
• Salmonella  (777152) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., Mortelmans, K., and Speck, W. Salmonella mutagenicity tests. III. Results from the testing of 225 chemicals Environ. Mutagen. Vol. 9 (Suppl 9) (1987) 1-109
  •  Negative 

Organ Systems Toxicity

• 27 WEEKS Continuous Breeding (Gavage)  (RACB90035) Completed 
  • NTIS No. PB91-211250
    Citation: Final Report on the Reproductive Toxicity of 4 Vinylcyclohexene in CD-1 Swiss Mice
  • M/F Mice: Swiss
  • Dose: N/A; 0,100,250,500 MG/KG/DAY; N/A; 0,500 MG/KG/DAY;
• SINGLE Total Reproductive Capacity (Intraperitoneal Injection)  (TRC98044) Completed 
  • F Mice: (SEC/E X C57BL/E)F1
  • Dose: 2000 MG/KG