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Optical Biosensor Support

Software

• For the determination of ligand distributions of different rate and affinity constants (MATLAB code) will be available soon
• For the microfluidics control of a Gilson syringe pump for small volume sample handling, contact Dr. Peter Schuck. 

Publications on Optical Biosensor Methodology

Cannon MJ, et al. Comparative analyses of a small molecule/enzyme interaction by multiple users of Biacore technology. Analytical Biochemistry 330:98-113, 2004.

Svitel J, Balbo A, Mariuzza RA, Gonzales NE and Schuck P. Combined affinity and rate constant distributions of analyte or ligand populations from experimental surface-binding kinetics and equilibria. Biophysical Journal 84:4062-4077, 2003.

Gilligan JJ, Schuck P, Yergey AL. Mass spectrometry after capture and small-volume elution of analyte from a surface plasmon resonance biosensor. Analytical Chemistry , 74:2041-2047, 2002.

Rebois V, Schuck P and Northup J. Elucidating kinetic and thermodynamic constants for the interaction of G protein subunits and receptors by surface plasmon resonance spectroscopy. Methods in Enzymology 344:15-42, 2002.

Gonzales NR, Schuck P, Schlom J and Kashmiri SVS. Surface plasmon resonance-based competition assay to assess the sera reactivity of variants of humanized antibodies. Journal of Immunological Methods 268:197-210, 2002.

Gorshkova II, Rausch JW, LeGrice SF, Crouch RJ. HIV-1 reverse transcriptase interactions with model RNA-DNA duplexes. Analytical Biochemistry 291:198-206, 2001.

Abrantes M, Magone MT, Boyd LF and Schuck P. Adaptation of a surface plasmon resonance biosensor with microfluidics for use with small sample volumes and long contact times. Analytical Chemistry 73:2828-2835, 2001.

Schuck P, Boyd LF and Andersen PS. Measuring protein interactions by optical biosensors. Current Protocols in Protein Science (Section 20.2.1-20.2.21), 1999.

Schuck P, Millar DB and Kortt AA. Determination of binding constants by equilibrium titration with circulating sample in a surface plasmon resonance biosensor. Analytical Biochemistry 265:79-91, 1998.

Schuck P. Methods for the reliable determination of antibody-antigen binding affinity and kinetics using surface plasmon resonance biosensors. Antibody Engineering II. IBC Library Series 946:95-113, 1997.

Schuck P. Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromolecules. Annual Reviews of Biophysics and Biomolecular Structure 26:541-566, 1997.

Schuck P. The reliable determination of binding kinetics and affinity using surface plasmon resonance biosensors. Current Opinion in Biotechnology 8(4):498-502, 1997.

Schuck P and Minton A. Kinetic analysis of biosensor data: Elementary tests for self-consistency. Trends in Biochemical Sciences 21:458-460, 1996.

Schuck P and Minton A. Analysis of mass transport limited binding kinetics in evanescent wave biosensors. Analytical Biochemistry 240:262-272, 1996.

Schuck P. Kinetics of ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor: I. A computer simulation of the influence of mass transport. Biophysical Journal 70:1230-1249, 1996.

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