Equipped with water-immersion lenses, an infra-red excitation source and a highly sensitive non-descanned detector, this instrument is oriented toward thick tissue sections and live animal imaging. A Meta detector allows for spectral imaging and online emission fingerprinting. |
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Microscope |
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Zeiss Axioskop 2 FS microscope (upright) |
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Objectives |
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5x Zeiss plan-apochromat dry, 0.16 NA |
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10x Zeiss achroplan water, 0.3 NA |
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20x Zeiss achroplan water, 0.5 NA |
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40x Zeiss achroplan, water, 0.8 NA, IR, DIC |
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63X Zeiss achroplan water, 0.9 NA, IR, DIC |
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Note: only two objectives are available on the microscope at any given time. Swapping objectives also requires software adjustment. Contact MIC staff. |
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Fluorescence Filters Cubes (visual inspection only) |
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FITC / GFP: 450-490 nm excitation, 515-586 nm emission. |
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Rhodamin: 540-552 nm excitation, emission beyond 590 nm. |
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DAPI: 350-400 nm excitation, 417-477 nm emission. |
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Note: the filter wheel also contains a cube used for IR excitation and non-descanned imaging. The DAPI cube may be swapped with the polarizer required for DIC imaging |
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Laser Lines (scanning only) |
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458, 488 and 514 nm: Argon ion laser, 30 mW |
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543 nm: Helium-Neon laser, 1mW |
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633 nm: Helium-Neon laser, 15mW |
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720-930 nm: tunable Chameleon IR laser, 1,870 mW max (2-photon excitation) |
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AOTF switched: FRAP, FRET and uncagging fully supported |
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Filter Blocks (scanning only, must be used in certain combinations) |
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Excitation: |
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NT 80/20: beam splitter, 20% excitation reflected, 80% emission transmitted |
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HFT UV/488/543/633: dichroic UV, 488, 543 and 633 nm |
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HFT KP 700/488: excitation above / emission below 700 nm + 488 nm dichroic |
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HFT KP 700/543: excitation above / emission below 700 nm + 543 nm dichroic |
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HFT 458/514: dichroic 458 and 514 nm |
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HFT 458: dichroic 548 nm |
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HFT 488: dichroic 488 nm |
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HFT KP 650: excitation above 650 nm, emission below 650 nm |
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Steering Meta vs PMT2/3: |
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NFT 490: long pass 490 nm |
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NFT 515: long pass 515 nm |
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NFT 545: long pass 545 nm |
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NFT 635 vis: long pass 635 nm |
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NFT KP 545: short pass 545 nm |
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Plate: glass plate correcting for misalignment, automatically inserted by software |
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Steering PMT 2 vs. PMT 3: |
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NFT 490: long pass 490 nm |
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NFT 545: long pass 545 nm |
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BG 39: IR blocking plate |
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Emission Filters |
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Meta detector |
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The Meta is a spectrometer spreading fluorescence emission among an array of detectors. It is, in itself, a configurable emission filter. |
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PMT 2 |
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KP 685: short pass 685 nm (IR laser) |
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BP 390-465 IR: band pass 390 to 465 nm, IR-blocking |
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BP 435-485 IR: band pass 435 to 485 nm, IR-blocking |
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BP 480-520 IR: band pass 480 to 520 nm, IR-blocking |
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BP 500-530 IR: band pass 500 to 530 nm, IR-blocking |
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BP 500-550 IR: band pass 500 to 550 nm, IR-blocking |
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LP 505: long pass 505 nm |
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PMT 3 |
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LP 560: long pass 560 nm |
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BP 500/20 IR: band pass 500 to 520 nm, IR-blocking |
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BP 500-550 IR: band pass 500 to 550 nm, IR-blocking |
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BP 535-590 IR: band pass 535 to 590 nm, IR-blocking |
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BP 565-615 IR: band pass 565 to 515 nm, IR-blocking |
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Detectors |
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Two photo-multiplier tubes fluorescence, 8 or 12 bit sampling (256 or 4096 gray levels, respectively) |
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One transmission detector for transmission / DIC imaging |
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One non-descanned detector for high-sensitivity (deep tissue) imaging |
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One Meta detector for spectral imaging. Up to 8 channels grouping any of the 32 detectors (10 nm each) may be defined (overlap between channels is not allowed). The Meta detector is almost as sensitive as a PMT except in the red, but twice as noisy. |
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Computer & Software |
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Fujitsu Siemens 2.4 GHz Xenon CPU running Windows 2000, 3.5 GB RAM, dual 114 GB storage, 2.4x DVD+/- recorder, dual 19" 1280 x 1024 flat panels |
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LSM 5 acquisition software with Physiology and Time Series modules |
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