Olympus TIRF Specifications
This Total Internal Reflection Fluorescence (TIRF) instrument is designed for high-speed acquisition of structures in close proximity to the glass-water interface. Note that temperature control is not yet fully supported.
Microscope | |
Olympus IX81 microscope (inverted) | |
Objective | |
60x PlanApo-N 1.45 NA, oil | |
Fluorescence Filters Cubes (visual inspection only) | |
FITC / GFP: 450-490 nm excitation, 515-586 nm emission. | |
Rhodamin: 540-552 nm excitation, emission beyond 590 nm. | |
DAPI: 350-400 nm excitation, 417-477 nm emission. | |
Laser Lines | |
458, 488, 514 nm: Argon ion laser, 200 mW | |
568, 647nm: Argon-Krypton ion laser, 81 mW | |
AOTF switched for rapid switching | |
Detector | |
Hamamatsu Orca C9100 backed-thinned camera, 512x512 pixels | |
Computer & Software | |
Macintosh G4 800 MHz 1.5GB ram | |
OpenLab acquisition package |