Cleaning Coverslips for Tissue Culture

Handle coverslips at all times with tweezers or gloved hands.

1. Place coverslips in Teflon racks and rinse for 1 min in dH2O with agitation.

2. Soak in 4N HCl for 3 days.

3. Rinse in dH2O.

4. Soak in 70% Ethanol overnight.

5. Rinse in dH2O.

6. Sterilize in the Teflon racks at 121°C for 20min sterilizing time and 20min exhaust time.