National Toxicology Program

CASRN: 50-55-5

Formula: C33-H40-N2-O9

Synonyms/Common Names:

• (3BETA,16BETA,17ALPHA,18BETA,20ALPHA)-11,17-DIMETHOXY-18-((3,4,5-TRIMETHOXYBENZOYL)OXY)-YOHIMBAN-16-CARBOXYLIC ACID METHYL ESTER

Known Uses:

ANTIHYPERTENSIVE DRUG

Short-Term Toxicity

• 2-WEEK (Dosed-Feed)  (C50157B) Completed 
  • Mice: MMTV/RAS (Tg.SH); Mice: MMTV/MYC (Tg.M); Mice: MMTV/NEU (Tg.Nk)
  • Dose: 0, 2.5, 5.0, 10.0, OR 20.0 PPM
• 14-Day (Dosed-Feed)  (C50157) Completed 
  • NTIS No. PB83-165761  (Peer Review 05/01/1979)
    Citation: Bioassay of Reserpine for Possible Carcinogenicity (CAS No. 50-55-5)
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Dosed-Feed)  (C50157) Completed 
  • NTIS No. PB83-165761  (Peer Review 05/01/1979)
    Citation: Bioassay of Reserpine for Possible Carcinogenicity (CAS No. 50-55-5)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: 0,.4,.8,1.5,3.0,6.0 PPM/10 PER GROUP

Long-Term Carcinogenicity

• 26&39-WK (Dosed-Feed)  (C50157B) Completed 
  • Mice: MMTV/RAS (Tg.SH); Mice: MMTV/MYC (Tg.M); Mice: MMTV/NEU (Tg.Nk)
  • Dose: MTD AND 1/2 MTD
• 2-Year (Dosed-Feed)  (C50157) Report Complete 
  • TR-193 (NTIS No. PB83-165761)  (Peer Review 05/01/1979)
    Bioassay of Reserpine for Possible Carcinogenicity (CAS No. 50-55-5)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Positive 
  • Female Rats Negative 
  • Male Mice Positive 
  • Female Mice Positive 
  • Dose: 0,5,10 PPM/50 PER GROUP

Genetic Toxicology

• Chromosome Aberrations  (013797) Completed 
  • Citation: Shelby, M.D. and Witt, K.L. Comparision of Results from Mouse Bone Marrow Chromosome Aberration and Micronucleus Test 25 (1995) Vol. 25, 1995 302-313
  • Mice
  • Chromosome Aberation  Positive 
• In Vitro Cytogenetics (CA/SCE)  (057864) Completed 
  • Citation: Gulati, D., Witt, K., Anderson, B., Zeiger, E.,and Shelby, M. Chromosome aberration and sister chromatid exchange test in Chinese hamster ovary cells in vitro III: Results with 27 chemicals Environ. Molec. Mutagen. Vol. 13 (1989) 133-193
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Negative 
• Drosophila  (531881) Completed 
  • Citation: Zimmering, S., Mason, J.M., and Valencia, R. Chemical mutagenesis testing in Drosophila. VII. Results of 22 coded compounds tested in larval feeding experiments. Environ. Molec. Mutagen. Vol. 14 (1989) 245-252
  • Reciprocal Translocation/Sex-Linked Recessive Lethal Inconclusive 
• Drosophila  (685541) Completed 
  • Citation: Foureman, P., Mason, J.M., Valencia, R., Zimmering, S. Chemical Mutagenesis Testing in Drosophila. X. Results of 70 coded compounds tested for the National Toxicology Program. Environ. Molec. Mutagen. Vol. 23 (1994) 208-227
  • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
• Mouse Lymphoma  (562201) Completed 
  • Citation: McGregor, D., Brown, A., Cattanach, P., Edwards, I., McBride, D., Riach, C., and Caspary, W. Responses of the L5178Y tk+/tk- mouse lymphoma cell forward mutation assay: III. 72 coded chemicals. Environ. Molec. Mutagen. Vol. 12 (1988) 85-154
  •  Negative 
• Micronucleus  (487993) Completed 
  • Citation: Shelby, M.D., Erexson, G.L., Hook, G.J., and Tice, R.R. Evaluation of a three-exposure mouse bone marrow micronucleus protocol: Results with 49 chemicals. Environ. Molec. Mutag. Vol. 21 (1993) 160-179
  • Mice: B6C3F1
  • Male Negative 
• Salmonella (NCI)  (N-9330) Completed 
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli Environ. Mutagen. Vol. 7 (Suppl 5) (1985) 1-248
  • Salmonella-NCI  Negative 
• Salmonella  (450632) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelmans, K. Salmonella mutagenicity tests. IV. Results from the testing of 300 chemicals Environ. Molec. Mutagen. Vol. 11 (Suppl 12) (1988) 1-158
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
  •  Negative 
• Sister Chromatid Exchanges  (013797) Completed 
  • Citation: Shelby, M.D. and Witt, K.L. Comparision of Results from Mouse Bone Marrow Chromosome Aberration and Micronucleus Test 25 (1995) Vol. 25, 1995 302-313
  • Mice
  • Sister Chromatid  Negative