Testing Information

Testing Status of Agents at NTP

Methylene chloride

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CASRN: 75-09-2

Formula: C-H2-CL2

Synonyms/Common Names:

  • DICHLOROMETHANE

Known Uses:

SOLVENT IN PAINT REMOVERS, DEGREASERS, AEROSOL PROPELLANTS, EXTRACTION SOLVENT IN FOOD PROCESSING; PHARMACEUTICAL AID, COMPONENT OF FIRE EXTINGUISHING CMPD (HSDB 1990)

Short-Term Toxicity

  • 13-Week (Inhalation)  (C50102B) Completed 
    • NTIS No. PB86-187903  (Peer Review 03/29/1985)
      Citation: Toxicology and Carcinogenesis Studies of Dichloromethane (Methylene Chloride) (CAS No. 75-09-2) in F344/N Rats and B6C3F1 Mice (Inhalation Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0,525,8400 PPM/10 PER GROUP

Long-Term Carcinogenicity

  • 2-YEAR (Inhalation)  (C50102B) Report Complete 
    • TR-306 (NTIS No. PB86-187903)  (Peer Review 03/29/1985)
      Toxicology and Carcinogenesis Studies of Dichloromethane (Methylene Chloride) (CAS No. 75-09-2) in F344/N Rats and B6C3F1 Mice (Inhalation Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Some Evidence 
      • Female Rats Clear Evidence 
      • Male Mice Clear Evidence 
      • Female Mice Clear Evidence 
    • Dose: R: 0,1000,2000,4000, M: 0,2000,4000 PPM/50 PER GROUP

Genetic Toxicology

  • In Vitro Cytogenetics (CA/SCE)  (676332) Completed 
    • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
    • Chromosome Aberation Negative 
    • Sister Chromatid Exchange Negative 
  • Mouse Lymphoma  (502376) Completed 
    • Citation: Myhr B.C., McGregor, D., Bowers, L., Riach, C., Brown, A., Edwards, I., McBride, D., Martin, R., and Caspary, W.J. L5178Y mouse lymphoma cell mutation assay results with 41 compounds. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 138-167
    •  Inconclusive 
  • Salmonella  (070295) Completed 
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Positive 
  • Salmonella  (413864) Completed 
    • Citation: Zeiger, E. Mutagenicity of 42 chemicals in Salmonella Environ. Molec. Mutagen. Vol. 16(Suppl 18) (1990) 32-54
    •  Negative 
  • Salmonella  (A34199) Completed 
    •  Positive 
  • Salmonella  (A86936) On Test 

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    Web page last updated on January 22, 2009