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pExpress-1 Vector Map

Below is a map of the pExpress-1 vector which was used to prepare specific XGC libraries based on the pExpress-1 library method overview.

pExpress1 Multiple Cloning Sites and Primer Binding Regions: 641-836

The sequence listed here is the (-) strand.

           700
•		  
  M13 pUC Forward 23-Base Sequencing PrimerT7 Promoter Primer
  5'-CC CAGTCACGAC GTTGTAAAAC G-3' 5'- TAATACGACT CACTATAGGG -3'
5'- GCAGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCTAGCTTA TAATACGACT CACTATAGGG ACCACTCCTC
 <------------T7 Promoter------------>  

 
cDNA Insert
  <-----------SP6 Promoter------------>
 GAGGTTAATTAAGCGGCCGCCCATCCCGGGAATTCCGGAGCGAAACTTGTTCTATTAGTGTCACCTAAATA
 XhoI       PacI           NotI           SmaI    EcoI           RsrII 3'-GATATCACA GTGGATTTA-5'
   SP6 Promoter Primer

 800
•		 
 GGCCTAATGGTCATAGCTCTGTTTCCTGTGTGAAATTGTTATCCGCT-3'
 3'-GGACACACTTTAACAATAGGCGA-'5
 M13/pUC 23-Base Sequencing Primer

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