High-Throughput Analysis of Subcellular Localization in Budding Yeast |
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| Launch in standalone player | |
| Air date: | Friday, February 06, 2004, 1:00:00 PM |
| Category: | Proteomics |
| Runtime: | 120 minutes |
| NLM Title: | High-throughput analysis of subcellular localization in budding yeast [electronic resource] / Erin O'Shea. |
| Series: | Global analysis of protein localization in budding yeast |
| Author: | O'Shea, Erin. National Institutes of Health (U.S.) |
| Publisher: | [Bethesda, Md. : National Institutes of Health, 2004] |
| Other Title(s): | Global analysis of protein localization in budding yeast |
| Abstract: | (CIT): A fundamental goal of cell biology is to define the functions of proteins in the context of compartments that organize them in the cellular milieu. We have constructed and analyzed a collection of yeast strains expressing full-length, chromosomally-tagged green fluorescent protein (GFP) fusion proteins. We classified these proteins, representing 75% of the yeast proteome, into twenty-two distinct subcellular localization categories and provide localization information for 70% of previously unlocalized proteins. Analysis of this high-resolution, high-coverage localization dataset in the context of transcriptional and protein-protein interaction data helps reveal the logic of transcriptional co-regulation and provides a comprehensive view of interactions within and between organelles in eukaryotic cells. For more information, visit the Proteomics Interest Group. |
| Subjects: | Gene Expression Regulation Protein Transport Proteomics Saccharomyces cerevisiae Proteins Saccharomyces cerevisiae |
| Publication Types: | Government Publications Lectures |
| Rights: | This is a work of the United States Government. No copyright exists on this material. It may be disseminated freely. |
| NLM Classification: | QU 58.5 |
| NLM ID: | 101268357 |
| CIT File ID: | 11812 |
| CIT Live ID: | 3063 |
| Permanent link: | http://videocast.nih.gov/launch.asp?11812 |