Our Science - Genetics/Genomics and DNA Sequencing
Core Genotyping Facility
Advanced Technology Center, NCI
Access: Open
High-throughput genotyping and sequencing
The aim of the Core Genotyping Facility (CGF) is to meet the genotyping and DNA sequencing needs of the NCI’s Division of Cancer Epidemiology and Genetics (DCEG) and Center for Cancer Research (CCR). The facility performs high-throughput genotyping and sequencing to support genetic analysis for a broad range of projects for the intramural research program of the NCI.
Contact: Meredith Yeager, PhD
E-mail: yeagerm@mail.nih.gov
Tel: 301-435-7613
DNA Sequencing MiniCore Facility
Laboratory of Experimental Carcinogenesis, CCR
Access: Open
Sample processing and sequencing and data analysis assistance
The DNA Sequencing MiniCore Facility accepts samples for DNA sequencing, or microsatellite analysis, from any investigator within the CCR. The facility analyzes these samples using state-of-the-art fluorescence-based sequencers. The MiniCore provides this service for the small-to-intermediate volume users. Most users submit 10 to 20 samples at a time. Emphasis is on analyzing samples rapidly and accurately. Most users receive data within one business day of submission. We also aid investigators in experimental design, interpretation of data, and trouble-shooting.
Contact: Mark J. Miller, PhD
E-mail: mark_james_miller@nih.gov
Tel: 301-496-5688, ext. 226
Comparative Molecular Cytogenetics Core Facility
Access: Open
Fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genome hybridization (CGH), chromosome flow sorting, karyotyping, and sister chromatid exchange (SCE)
The Comparative Molecular Cytogenetic Core provides molecular cytogenetic support services to the NCI community. This facility aids in the assessment of structural and numerical genomic changes in cancer, pre-cancer, and cancer models. In general, services are provided for cytogenetic analyses, which are not routinely available from extramural providers. The core is the only facility in the NIH with chromosome flow sorting capabilities and has particular expertise in the cytogenetics of animal models including, but not limited to, murine and non-human primates species.
Contact: Sandra Burkett
E-mail: sburkett@ncifcrf.gov
Tel: 301-846-1262
