Mechanisms of Dopamine Transmission in the VTA |
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| Air date: | Monday, September 08, 2008, 12:00:00 PM |
| Category: | Neuroscience |
| Runtime: | 75 minutes |
| NLM Title: | Mechanisms of dopamine transmission in the VTA [electronic resource] / John Williams. |
| Author: | Williams, John. National Institutes of Health (U.S.) |
| Publisher: | [Bethesda, Md. : National Institutes of Health, 2008] |
| Abstract: | (CIT): Dendritic release of dopamine in the ventral tegmental area acts on D2-dopamine receptors to mediate an inhibitory postsynaptic current (IPSC). This study examines how the rise and fall in extracellular dopamine regulates the kinetics of the IPSC. Extracellular dopamine was measured electrochemically during simultaneous recording of the IPSC. Both the rise and fall of dopamine were rapid relative to the IPSC, suggesting that G-protein dependent potassium channel activation determined the time course of the IPSC. The kinetics of D2 receptor dependent activation of potassium currents was studied using outside-out patch recordings and rapid application of dopamine. Dopamine applied at a minimum concentration of 10 ℗æM for a maximum of 100 ms mimicked the IPSC. Shorter applications of higher concentrations did not change the kinetics of the outward current. These measurements were used to construct a model predicting that dopamine travels less then 500 nm from the site of release before binding to D2 receptors. |
| Subjects: | Dopamine--physiology Receptors, Dopamine D2 Ventral Tegmental Area |
| Publication Types: | Government Publications Lectures |
| Download: | Download
Video How to download a Videocast |
| NLM Classification: | WK 725 |
| NLM ID: | 101486619 |
| CIT File ID: | 14626 |
| CIT Live ID: | 7090 |
| Permanent link: | http://videocast.nih.gov/launch.asp?14626 |
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Enhanced Audio Podcast | 55:46 |
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Enhanced Video Podcast | 55:46 | |