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Protocols

Immunohistochemistry

Manual Tissue Staining Protocols(www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/protocols.cfm#manual)
Automatic Tissue Staining Protocols(www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/protocols.cfm#auto)
Manual Cell Staining Protocols(www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/protocols.cfm#cell)

The Immunohistochemistry staff provides protocols for tissue staining. Please note that protocols are continually updated and primary antibody dilutions may change with different lot numbers. For assistance with a specific protocol, please contact Natasha Clayton(clayton@niehs.nih.gov). All protocols are in Adobe PDF format. Adobe Acrobat Reader(http://www.adobe.com/products/acrobat/readstep2.html) is required to view the protocol files.

Manual Tissue Staining Protocols
Antibody	Fixative	Paraffin/ Frozen	Species	Images
Ah Receptor	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ahrec-r.pdf) (42 KB)
Amylase	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/amylase-r.pdf) (36 KB)
APC	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/apc.pdf) (45 KB)
BAF170	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/baf170-h.pdf) (36 KB)
B-Catenin *	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/bcatenin-m.pdf) (43 KB)
Beta Polymerase	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/betapoly.pdf) (26 KB)
BRDU *	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/brdu.pdf) (78 KB)
Calbindin D	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/calbindin-d-m.pdf) (97 KB)
Calgranulin	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/calgranulin.pdf) (37 KB)
Calretinin	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/calretinin.pdf) (44 KB)
Caspase-3 *	Formalin	Paraffin	Rat/Mouse/Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/caspase3.pdf) (73 KB)
Catalase	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/catalase-r.pdf) (37 KB)
CC10	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cc10-r.pdf) (36 KB)
CCK	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cck.pdf) (43 KB)
CCK-A Receptor	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cck-ar.pdf) (49 KB)
CD19 *	N/A	Frozen	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd19frozmse.pdf) (33 KB)
CD25	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd25-h.pdf) (42 KB)
CD3 *	Formalin	Paraffin	Human/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd3.pdf) (43 KB)
CD34	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd34-m.pdf) (38 KB)
CD4	N/A	Frozen	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd4-frozen-m.pdf) (34 KB)
CD4	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd4-h.pdf) (36 KB)
CD-40	N/A	Frozen	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd40-frozen-m.pdf) (34 KB)
CD-40	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd40-m.pdf) (36 KB)
CD45	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd45-m.pdf) (40 KB)
CD8	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd8-h.pdf) (38 KB)
CD8a	N/A	Frozen	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cd8a-frozen-m.pdf) (39 KB)
Chromogranin A	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/chromoa-m.pdf) (105 KB)
Chromogranin A	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/chromoa-r.pdf) (105 KB)
Clusterin	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/clusterin-m.pdf) (43 KB)
Clusterin	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/clusterin-h.pdf) (41 KB)
Cox1	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cox-1-m.pdf) (99 KB)
Cox2 *	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cox-2-m.pdf) (37 KB)
Cox2 *	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cox-2-r.pdf) (37 KB)
CXCL12	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cxcl12-h.pdf) (98 KB)
Cyclin D1	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyclin-d1-m.pdf) (99 KB)
Cyclin D1	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyclin-d1-r.pdf) (37 KB)
Cyp1A1	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp1a1-r.pdf) (98 KB)
Cyp1A2	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp1a2-r.pdf) (36 KB)
Cyp2B1/2	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp2b1-2.pdf) (44 KB)
Cyp2C11	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp2c11-r.pdf) (37 KB)
Cyp2C12	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp2c12-r.pdf) (38 KB)
Cyp2D1	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp2d1-r.pdf) (37 KB)
Cyp2E1	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp2e1-r.pdf) (37 KB)
Cyp3A1	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp3a1-r.pdf) (36 KB)
Cyp3A2	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp3a2-r.pdf) (37 KB)
Cyp4A	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cypa4-mouse.pdf) (35 KB)
Cyp4A	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyp4a-rat.pdf) (36 KB)
Cytokeratin 5	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyto5-mouse.pdf) (102 KB)
Cytokeratin 8	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyto8-rat.pdf) (40 KB)
Cytokeratin 14	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyto14-rat.pdf) (102 KB)
Cytokeratin 15	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyto15-mouse.pdf) (36 KB)
Cytokeratin 15	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/keratin15lsab.pdf) (43 KB)
Cytokeratin 20	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyto20-rat.pdf) (41 KB)
Cytokeratin Wide Spectrum *	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cytowidemouse.pdf) (94 KB)
Cytokeratin Wide Spectrum *	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cytowiderat.pdf) (96 KB)
5mCytidine	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/5mcytidine.pdf) (36 KB)
Desmin	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/desmin-mouse.pdf) (35 KB)
Desmin	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/desmin-rat.pdf) (36 KB)
Dnmt3b	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/dnmt3b-mouse.pdf) (36 KB)
Dss1	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/dss1.pdf) (42 KB)
E-cadherin	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ecadherin.pdf) (36 KB)
ED2 *	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ed2.pdf) (92 KB)
eNOS	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/enos.pdf) (45 KB)
EphA3	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/epha3.pdf) (29 KB)
Estrogen Receptor Alpha	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/estrogen.pdf) (36 KB)
Estrogen Receptor Alpha	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/estrogen-rat.pdf) (37 KB)
Estrogen Receptor Beta	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/erbetarat.pdf) (40 KB)
F4/80 *	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/f480.pdf) (85 KB)
Factor 8 *	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/factor8.pdf) (38 KB)
Factor 8 *	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/factor8_human.pdf) (102 KB)
FAS-L	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/fasl.pdf) (36 KB)
FLAG	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/flag.pdf) (39 KB)
FOXP3	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/foxp3.pdf) (40 KB)
GFAP	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/gfapmouse.pdf) (38 KB)
GFAP	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/gfaprat.pdf) (38 KB)
Glucagon	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/glucagon-mse.pdf) (35 KB)
Glucagon	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/glucagon-rat.pdf) (35 KB)
GSTpi	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/gstpi.pdf) (34 KB)
H2A.X	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/h2a-x.pdf) (42 KB)
HA11	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ha11.pdf) (37 KB)
HDAC2	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/hdac2-human.pdf) (28 KB)
Histone H2B (Ser14)	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/histone-ser14.pdf) (43 KB)
Histone H2B (Lys5)	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/histoneh2blys5.pdf) (40 KB)
Histone H2B (Lys15)	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/histone-lys15.pdf) (46 KB)
Histone H3 (Lys18)	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/histone-lys18.pdf) (44 KB)
Histone H3 (Ser10)	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/histone-ser10.pdf) (36 KB)
Hsp-25	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/hsp25elite.pdf) (37 KB)
ICAM	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/icam.pdf) (47 KB)
IGF-1	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/igf1.pdf) (37 KB)
IGF-beta	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/igf1beta.pdf) (42 KB)
IL1 alpha	PLP	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/il1alphaplp.pdf) (13 KB)
IL1 beta	PLP	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/il1betaplp.pdf) (32 KB)
iNOS	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/inos.pdf) (36 KB)
Insulin	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/insulin.pdf) (16 KB)
Insulin	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/insulin-mom.pdf) (27 KB)
Keratin 1	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyto1-mouse.pdf) (101 KB)
Keratin 10	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cyto10-mouse.pdf) (99 KB)
Ki67	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ki67rat.pdf) (46 KB)
Ki67 *	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ki67mouse.pdf) (45 KB)
MAdCAM-1	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/madcam1_mse.pdf) (39 KB)
Marck's Protein	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/marcks.pdf) (15 KB)
MEK-1	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/mek-1_mse.pdf) (36 KB)
Metallothionein	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/metallothionein_rat.pdf) (39 KB)
MMP-9	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/mmp9.pdf) (29 KB)
Mouse IgG	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/mouse-ig.pdf) (14 KB)
Mucin 2	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/mucin2-human.pdf) (36 KB)
Mucin-5AC	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/mucin-5ac-mse.pdf) (100 KB)
Neomycin	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/neomycin.pdf) (43 KB)
NFkappa B	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/nfk.pdf) (50 KB)
Neurofilament	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/neurofilament.pdf) (43 KB)
Neuron Specific Enolase (NSE)	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/nse2rat.pdf) (43 KB)
Neuron Specific Enolase (NSE)	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/nse2mouse.pdf) (46 KB)
Oct-4	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/oct-4-human.pdf) (30 KB)
p15	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/p15.pdf) (44 KB)
p19arf	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/p19arf.pdf) (37 KB)
p21	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/p21.pdf) (40 KB)
p53	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/p53human.pdf) (40 KB)
p53 *	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/p53rodent.pdf) (40 KB)
p63	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/p63-mse.pdf) (29 KB)
Pan-MCM	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/panmcm.pdf) (16 KB)
Pancreatic Polypeptide	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pancreatic-poly.pdf) (106 KB)
Pax-5	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pax-5_mse.pdf) (39 KB)
Pax-5	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pax-5_rat.pdf) (30 KB)
PKC-Theta	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pkctheta.pdf) (47 KB)
PCNA *	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pcna.pdf) (33 KB)
Pecam (CD31)	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pecamrat.pdf) (40 KB)
Pecam (CD31)	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pecammse.pdf) (40 KB)
PNA	Frozen	N/A	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pna.pdf) (29 KB)
Progesterone Receptor	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/progesterone.pdf) (37 KB)
S100	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/s100rat.pdf) (38 KB)
S100	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/s100mouse.pdf) (27 KB)
Smooth Muscle Actin	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/sma-tissue.pdf) (26 KB)
Somatostatin	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/somatostatin-rat.pdf) (14 KB)
Somatostatin	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/somatostatin-mouse.pdf) (105 KB)
SP-A	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/sparat.pdf) (44 KB)
SP-A	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/spamouse.pdf) (46 KB)
Superoxide Dismutase 2	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/sod2-rat.pdf) (28 KB)
SV40	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/sv40tag.pdf) (14 KB)
Synaptophysin	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/synap.pdf) (97 KB)
Thrombospondin	Formalin	Paraffin	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/tsp.pdf) (39 KB)
Thyroglobulin	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/thyroglobulin.pdf) (35 KB)
TNF alpha PLP	PLP	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/tnfalphaplp.pdf) (13 KB)
TNF alpha frozen	N/A	Frozen	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/tnf-frozen.pdf) (14 KB)
VCAM	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/vcam.pdf) (42 KB)
VegF	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/vegf.pdf) (42 KB)
Vimentin Rat *	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/vimentin.pdf) (33 KB)
Vimentin Mouse *	Formalin	Paraffin	Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/vimentinmouse.pdf) (36 KB)
Vitamin D	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/vitamind.pdf) (44 KB)
WT1	Formalin	Paraffin	Rat/Mouse (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/wt1.pdf) (44 KB)
Ventana (Automatic Stainer)
Antibody	Fixative	Paraffin/ Frozen	Species	Images
Beta Tubulin	Formalin	Paraffin	Rodent (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/betatube.pdf) (43 KB)
CCSP	Formalin	Paraffin	Rodent (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ccsp.pdf) (44 KB)
Chromogranin A			Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/chromoa.pdf) (13 KB)
CK34betaE12	N/A	Frozen	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/ck34betae12.pdf) (9 KB)
KAI-1	N/A	Frozen	Human (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/kai1.pdf) (12 KB)
MCM	Formalin	Paraffin	Rat (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/mcm.pdf) (13 KB)
Manual Cell Lines Staining Protocols
Cell Pellet Protocol (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/pellet.pdf) (43 KB)
Cytokeratin Wide Spectrum (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/cytokeratinws.pdf) (28 KB)
Myosin (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/myosinheavychaincells.pdf) (45 KB)
Smooth Muscle Actin (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/smacells.pdf) (25 KB)
Vimentin (http://www.niehs.nih.gov/research/atniehs/labs/lep/path-support/immuno/docs/vimentincells.pdf) (27 KB)

* Routine antibodies performed by the Immunohistochemistry Lab.

111 T.W. Alexander Drive, Research Triangle Park, NC USA 27709

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Last Reviewed: October 30, 2008