Release of a 7.6-Fold Sequence Genome Assembly and 207,000 EST Sequences of Aedes aegypti

The National Institute of Allergy and Infectious Diseases, National Institutes of Health (NAID/NIH) has funded the sequencing, assembly, and annotation of the Aedes aegypti genome through its Microbial Sequencing Centers, the Institute for Genomic Research, and the Broad Institute, Massachusetts Institute of Technology. Whole genome shotgun sequence data have now been generated and assembled, providing a 7.6X coverage of the Aedes genome, which is estimated to be 1.383Gb in length. This Whole Genome Shotgun project has been deposited at GenBank under the project accession AAGE00000000. The version described in this annoucement is the second version, AAGE02000000.

Assembly Statistics

• Total number of sequence reads: 14.9 million
• Total number of sequence reads placed in the assembly: 12.3 million
• Average contig coverage: 7.6X (6.71X in Q20 bases)
• Number of contigs: 36,206
• Total length of combined contigs 1.31 Gb
• Number of scaffolds: 4,758
• Total length of combined scaffolds (including gaps) 1.383 Gb

Additional information about the Aedes aegypti sequencing project and assembly can be found at the Broad Institute http://www.broad.mit.edu/annotation/disease_vector/aedes_aegypti/ and at TIGR http://www.tigr.org/msc/aedes/aedes.shtml.

Annotation Release

A preliminary annotation of the assembly is expected to be released in December 2005 in VectorBase, a NIAID-funded Bioinformatics Resource Center for invertebrate vectors of human pathogens, at http://www.vectorbase.org. A more complete annotation, including functional assignments and gene accessions that will be tracked forward, will be released in Genbank and VectorBase in the spring of 2006. Long-term curation of the sequence and subsequent annotation updates will be the responsibility of VectorBase.

Supplementary Data Release

Complementary to the sequencing and annotation project described above, NAID/NIH has also funded a project to provide additional Aedes aegypti genome-related resources. As part of this project The Institute of Genomic Research has released in Genbank 207,079 Aedes aegypti EST sequences and 117,937 BAC end sequences generated from a variety of libraries. The EST accession ranges to date are DV230611-DV235882, DV235883-DV270715, DV300289-DV348598, DV348687-DV389983, DV389984, and DV437762. The BAC end sequence accession ranges are CC065891-CC144307, CC149837-CC155974, and CC841856-CC875159.

These sequences are also available as part of the TIGR Aedes aegypti gene index at The Institute for Genomic Research—Gene Indices.

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