Input Sequences Search limit: 10 sequences Time limit: 1 hour per submission Upload local file or Enter text or Load example —— AA Examples —— ms1.aa ngts.aa gtm1_human.aa protease-4x300 Fasta Options (options details) Protein Database: Genpept Genpept_new Swissprot Protein Data Bank Refseq human Refseq mouse Yeast Drosophila E. Coli Mitochondrial Comparison Matrix: -s Blosum50 Blosum62 Blosum80 PAM 250 PAM 120 PAM 40 PAM 20 MDM_10 MDM_20 MDM_40 Word size: 1 2 Statistics: -z NO statistics regression vs length of the library use the ln() length correction estimates based on BLOSUM scoring matrices and gap penalties an alternate regression method Gap penalties: -f first residue -g additional residues Limit results: -b best scores -d best alignments -F < score < -E Output format: -m identity=: conservative replacements=. conservative replacements=x' non-conservative ='X' Only show differences Aligned database sequence only Graphical display of the boundaries of the alignments Parseable format -1 sort by 'init1' no sort
Protein Database: Genpept Genpept_new Swissprot Protein Data Bank Refseq human Refseq mouse Yeast Drosophila E. Coli Mitochondrial Comparison Matrix: -s Blosum50 Blosum62 Blosum80 PAM 250 PAM 120 PAM 40 PAM 20 MDM_10 MDM_20 MDM_40 Word size: 1 2 Statistics: -z NO statistics regression vs length of the library use the ln() length correction estimates based on BLOSUM scoring matrices and gap penalties an alternate regression method Gap penalties: -f first residue -g additional residues Limit results: -b best scores -d best alignments -F < score < -E Output format: -m identity=: conservative replacements=. conservative replacements=x' non-conservative ='X' Only show differences Aligned database sequence only Graphical display of the boundaries of the alignments Parseable format -1 sort by 'init1' no sort