DNA Minicore: A Small, Localized DNA Sequencing Core Facility
Research: The mini-DNA sequencing core facility is designed to
provide investigators of the Center for Cancer Research with rapid
and accurate sequencing of DNA samples. The facility has been in
operation since April 1997 and has processed over 65,000 samples
submitted by over 390 investigators in 24 separate laboratories.
Sequences are determined on ABI Prism 377 or 3100 automatic sequencers.
In addition, computer equipment and software is provided to aid investigators
in analysis of data. The facility provides expertise in the use of
this software as well as consulting in experimental design and help
in troubleshooting sequencing reactions. A major emphasis has been
placed on maximizing the use of the sequencer and minimizing the
time between sample submission and processing. We accept samples
where the reaction has been done by the users as well as samples
that are ready for the sequencing reaction. As many as 300 samples
can be run per day. Nevertheless, the average turnover time has steadily
decreased over the years and is currently less than one day. A locally
developed database helps us to keep track of sample submission and
processing, the order in which samples may most efficiently be processed
and the costs. It is also used to control distribution of the finished
data sets and to inform users that their sample data is ready for
analysis.
Use of the DNA Minicore has grown at a rate close to 50% per year
for several years. Nevertheless, we have been able to function
as if we were a small localized facility catering to only a few users.
This is due to the highly competent technical staff assigned to
the
facility and their dedication. In particular, we can tailor electrophoresis
runs to the needs of an investigator for a particular project,
including microsatellite analysis. We expect that this facility will
represent
a model for future small, localized core facilities providing cost-effective
support for investigators at the NIH.
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