OVERVIEW

The CCR Office of Science and Technology Partnerships (OSTP) and the NCI Research Contracts Branch have negotiated a partnership with Myriad Genetics for the discovery of novel protein-protein interactions and the delineation of biological association networks. This program involves an Indefinite Delivery/Indefinite Quantity contract to access Myriad's automated process for the large-scale identification of protein-protein interactions that is based on the Yeast Two-Hybrid (Y2H) methodology . The program was launched during the week of September 20, 2004, as 20 Principal Investigators submitted a total of 37 genes of interest that will be subjected to comprehensive Yeast two-hybrid screens against three activation domain libraries. The program is currently being partially subsidized  50% by the CCR OSTP.  Currently, over 45 CCR investigators have participated in this program to identify binding partners of nearly 100 cancer- and other disease-associated proteins of interest to the CCR community.  

Myriad has assigned a senior scientist, Dr. Yang Chen, to work with each investigator to help define strategies (selection of bait constructs, Activation Domain libraries, etc.) that will be used in the analysis. Dr. Chen,  and her supervisor, Dr. Donna Shattuck, Vice President of Population Genetics, both have had a long-standing interest in cancer research and have collectively been at Myriad nearly two decades.  

Instructions for requesting yeast 2-hybrid screens:  T o discuss a new project and to develop a screening strategy, please contact Dr. Chen directly through her contact information shown below.  Once you have determined the number of baits to be screened, please visit the OSTP subsidy request site ( https://ccrod.cancer.gov/Subsidy/ ) to submit a request for supplemental funding from the OSTP.  On the Request Form, please select "protein-protein interaction screens" under the service type drop-down menu, and select "Myriad" under the Service Company drop-down menu.  Please also indicate the name of your Administrative Officer, including their email address, and the gene name and the number of baits to be screened.

The data generated by Myriad screens is provided to CCR scientists through the NCI ProNet web site described on the Data Management Page .  Positive and negative search results will be provided to you through this site. This information can be used to more efficiently direct additional Y2H searches.   To more effectively share and communicate information related to these genes and the interaction data generated through the Myriad partnership, the OSTP and the Advanced Biomedical Computing Center (ABCC) has launched a new web site , the CCR Protein Interaction Data Exchange , or C-PrIDE.  

• Browse a list of all genes (baits) submitted by CCR investigators to Myriad for yeast 2-hybrid screening - A great way to discover what genes your colleagues are studying;
• Access a host of unpublished protein interactions (preys) identified through Myriad's yeast 2-hybrid technology;
• Link directly from C-PrIDE gene listings to a variety of publicly available gene and protein-related databases, including EntreGene, Biocarta, GeneCards, GO database, etc.;
• Search the entire C-PrIDE database using common gene names or aliases to find out if they were pulled out as "preys" in one of Myriad's Y2H screens;
• Browse a comprehensive listing of signal transduction genes (281 baits!) and associated protein interaction data (mostly unpublished prey) generated by Myriad for the Alliance for Cellular Signaling (AfCS: http://www.signaling-gateway.org/);
• Link directly to contact information for individual participants in the program to inquire about the genes that were screened (baits) and their binding partners (preys); and
• View and post validation data confirming yeast 2-hybrid interactions in mammalian cells. 

MYRIAD GENETICS Y2H CONTACT INFORMATION

Donna Shattuck, Ph.D.
VP Population Genetics
Myriad Genetics
320 Wakara Way
Salt Lake City, UT 84108
donna@myriad.com
801 584-3629

Yang Chen, Ph.D.
Project Director
Myriad Genetics
320 Wakara Way
Salt Lake City, UT 84108
chenya@myriad.com
801 584-3554

To inquire about participating in the Yeast 2-Hybrid program, please contact one of the OSTP staff