FEBS Letters 448: 269-272

Recognizing the Pleckstrin homology domain fold in mammalian phospholipase D using hidden Markov models

Pamela G. Holbrook^a, V. Geetha^b, Michael A. Beaven^a, Peter J. Munson^b

^a Laboratory of Molecular Immunology, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda MD 20892, USA
^bAnalytical Biostatistics Section, Mathmatecal and Statistical Computing Laboratory, Center for Information Technology, National Institutes of Health, Building 12A, Room 2041, Bethesda, MD 20892, USA

Abstract

Phospholipase D was first described in plant tissue but has recently been shown to occur in mammalian cells where it is activated by cell surface receptors. Its mode of activation by receptors is unclear. Biochemical studies suggest that it may occur downstream of other effector proteins and that small GTP-dependent regulatory proteins may be involved. The sequence in a non-designated region of mammalian phospholipase D1 and 2 shows similarity to a structural domain that is present in signalling proteins that are regulated by protein kinases or heterotrimeric G-proteins. Mammalian phospholipase D has structural similarities with other lipid signalling phospholipases and thus may be regulated by receptors in an analogous fashion.

Recognizing the Pleckstrin homology domain fold in mammalian phospholipase D using hidden Markov models

Pamela G. Holbrooka, V. Geethab, Michael A. Beavena, Peter J. Munsonb

Abstract

Pamela G. Holbrook^a, V. Geetha^b, Michael A. Beaven^a, Peter J. Munson^b