More on Affymetrix : NIDDK

Scientific Programs

Different types of array used in the lab:

C.elegans Genome Array

Drosophila Genome 2.0 Array

E.coli Genome 2.0 Array

Human Genome U133 Plus 2.0

Mapping 500K Array (hyb+stain)

Mouse Genome 430 2.0

Mouse Genome 430A 2.0

Mouse Tiling 2.0 R Array (hyb+stain)

Mouse Exon 1.0 ST Array (hyb+stain)

Rat Genome 230 2.0

Rhesus macaque Genome

Yeast genome 2.0

Yeast genome S98

Genechip Custom Array (hyb+stain)

More on Affymetrix

Instruments and Software used are:

GeneChip Hybridization Oven 640

GeneChip Hybridization Oven 320

GeneChip Fluidics Station 450

GeneChip Scanner 3000 7G with AutoLoader- can hold 48 arrays at a time, enabled for High-Resolution Scanning.

GeneChip Operating Software (GCOS) v1.1.1

Affymetrix

Technical Documentation: http://www.affymetrix.com/support/technical/index.affx
Manuals : http://www.affymetrix.com/support/technical/manuals.affx
Technical Notes: http://www.affymetrix.com/support/technical/technotesmain.affx
Data Analysis: https://www.affymetrix.com/support/downloads/manuals/data_analysis_fundamentals_manual.pdf (log-in required) 
NetAffx: http://www.affymetrix.com/analysis/index.affx (log-in required)

Microarray Sample Processing Workflow




The concentration of the samples are deduced using the Nanodrop, and the RNA integrity is shown using the Bioanalyzer. The samples are then processed through varies steps including IVT labeling, Hybridization and Scanning. The raw data (Image files) is analyzed and packaged by our bioinformatics group.

Page last updated: January 01, 0001

General inquiries may be addressed to: Office of Communications & Public Liaison
NIDDK, NIH
Building 31. Rm 9A06
31 Center Drive, MSC 2560
Bethesda, MD 20892-2560
USA
For information about NIDDK programs: 301.496.3583

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