Judah L. Rosner, Ph.D. : NIDDK

Judah L. Rosner, Ph.D.


LMB
MOLECULAR GENETICS SECTION
NIDDK, National Institutes of Health
Building 5, Room 333
5 Memorial Dr.
Bethesda, MD 20892-0560
Tel: 301-496-5466
Fax: 301-496-0201
Email: jlrosner@helix.nih.gov

Education / Previous Training and Experience:
B.A., Columbia College, 1960
M.A., Yale University, 1964
Ph.D., Yale University, 1967


Research Statement:

Our major research interest is to understand how bacterial gene expression is regulated, particularly genes that engender multiple antibiotic resistance. Specifically, we are studying the mechanism of transcriptional activation by MarA, SoxS and Rob, a homologous group of proteins that stimulate the expression of more than 25 genes involved in resistance to diverse antibiotics, superoxides, and organic solvents in Escherichia coli. By combining genetics, molecular biology, and crystallography, we are learning how these proteins are regulated and how they bind to DNA and interact with RNA polymerase to activate transcription.



Selected Publications:

Kawano M, Storz G, Rao BS, Rosner JL, Martin RG Detection of low-level promoter activity within open reading frame sequences of Escherichia coli. Nucleic Acids Res (33): 6268-76, 2005. [Full Text/Abstract]

Thomason LC, Court DL, Datta AR, Khanna R, Rosner JL Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase. J Bacteriol (186): 8248-53, 2004. [Full Text/Abstract]

Martin RG Rosner JL Transcriptional and translational regulation of the marRAB multiple antibiotic resistance operon in Escherichia coli. Mol Microbiol (53): 183-91, 2004. [Full Text/Abstract]

Dangi B, Gronenborn AM, Rosner JL, Martin RG Versatility of the carboxy-terminal domain of the alpha subunit of RNA polymerase in transcriptional activation: use of the DNA contact site as a protein contact site for MarA. Mol Microbiol (54): 45-59, 2004. [Full Text/Abstract]

Martin RG Rosner JL Analysis of microarray data for the marA, soxS, and rob regulons of Escherichia coli. Methods Enzymol (370): 278-80, 2003. [Full Text/Abstract]

Martin RG Gillette WK Martin NI Rosner JL Complex formation between activator and RNA polymerase as the basis for transcriptional activation by MarA and SoxS in Escherichia coli. Mol Microbiol (43): 355-70, 2002. [Full Text/Abstract]

Martin RG Rosner JL Genomics of the marA/soxS/rob regulon of Escherichia coli: identification of directly activated promoters by application of molecular genetics and informatics to microarray data. Mol Microbiol (44): 1611-24, 2002. [Full Text/Abstract]

Rosner JL Dangi B Gronenborn AM Martin RG Posttranscriptional activation of the transcriptional activator Rob by dipyridyl in Escherichia coli. J Bacteriol (184): 1407-16, 2002. [Full Text/Abstract]

Martin RG Rosner JL The AraC transcriptional activators. Curr Opin Microbiol (4): 132-7, 2001. [Full Text/Abstract]

Martin RG Gillette WK Rosner JL Promoter discrimination by the related transcriptional activators MarA and SoxS: differential regulation by differential binding. Mol Microbiol (35): 623-34, 2000. [Full Text/Abstract]

Martin RG Gillette WK Rhee S Rosner JL Structural requirements for marbox function in transcriptional activation of mar/sox/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter. Mol Microbiol (34): 431-41, 1999. [Full Text/Abstract]

Martin RG, Gillette WK, Rhee S, Rosner JL Structural requirements for marbox function in transcriptional activation of mar/sox/rob regulon promoters in Escherichia coli: sequence, orientation and spatial relationship to the core promoter. Mol Microbiol (34): 431-41, 1999. [Full Text/Abstract]

Rhee S Martin RG Rosner JL Davies DR A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator. Proc Natl Acad Sci U S A (95): 10413-8, 1998. [Full Text/Abstract]

Martin RG, Rosner JL Fis, an accessorial factor for transcriptional activation of the mar (multiple antibiotic resistance) promoter of Escherichia coli in the presence of the activator MarA, SoxS, or Rob. J Bacteriol (179): 7410-9, 1997. [Full Text/Abstract]

Martin RG, Jair KW, Wolf RE Jr, Rosner JL Autoactivation of the marRAB multiple antibiotic resistance operon by the MarA transcriptional activator in Escherichia coli. J Bacteriol (178): 2216-23, 1996. [Full Text/Abstract]

Martin RG, Rosner JL Binding of purified multiple antibiotic-resistance repressor protein (MarR) to mar operator sequences. Proc Natl Acad Sci U S A (92): 5456-60, 1995. [Full Text/Abstract]

Cohen SP, Levy SB, Foulds J, Rosner JL Salicylate induction of antibiotic resistance in Escherichia coli: activation of the mar operon and a mar-independent pathway. J Bacteriol (175): 7856-62, 1993. [Full Text/Abstract]



Page last updated: December 17, 2008

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