Based on the mapping of the human genome and the development
of information databases, a broad description of genes transcribed
in blood cells is now known. Hembase was developed to provide
worldwide access to those genetic-based studies performed
by scientists in the Molecular Biology and Genetics Section,
Molecular Medicine Branch, Division of Intramural Research,
National Institute of Diabetes and Digestive and Kidney Diseases
(NIDDK).
This project represents the shared goal of several individuals
and groups (credits)
interested in disseminating genomic information on the World
Wide Web.
Hembase Focus: Red Cells
The production of red blood cells occurs
by a process called erythropoiesis whereby erythroid
progenitor cells proliferate and differentiate into
erythroid precursor cells (see figure above). Normally,
this process is highly dependent upon and regulated
by a hormone produced by the kidneys called erythropoietin.
Our laboratory investigates genes transcribed in human
erythroid cells as they differentiate into mature red
blood cells. Red blood cells have the fundamental role
of delivering oxygen from the lungs to the other body
tissues, and diseases associated with erythroid cells
affect millions of people. We hope this data will be
useful for the understanding and improved treatment
or prevention of those diseases including all forms
of anemia, erythroleukemia, and malaria.
Hembase Content
Red blood cells in the circulation arise from hematopoietic stem cells that proliferate as erythroid progenitors and differentiate into erythroid precursor cells in response to the hormone erythropoietin. Messenger RNA was isolated from those cells and used to generate gene libraries. Sequencing several thousand expressed sequence tags (EST) from those libraries was then performed. Those EST and sequences encoding several hundred additional genes with known expression in erythroid cells are compiled here as a database of human erythroid gene activity. The database is organized and linked according to the location of these sequences within the human genome.