High-Speed Parallel Molecular Nucleic Acid Sequencing
Background:
The NCI Nanobiology Program is
seeking statements of capability or interest from parties
interested in collaborative research to further develop, evaluate,
or commercialize nanoscale or molecular nucleic acid sequencing.
The IP is also available for co-exclusive
licensing.
Technology:
The technology is a new system,
methods and compositions for DNA sequencing, also known as Two Dye
Sequencing (TDS). This invention is based on Fluorescence Resonance
Energy Transfer (FRET), a technology increasingly in use for
several molecular analysis purposes. In particular, the method
consists of: 1) attachment of engineered DNA polymerases labeled
with a donor fluorophore to the surface (chamber) of a microscope
field of view, 2) addition to the chamber of DNA with an annealed
oligonucleotide primer and template, which is bound by the
polymerase, 3) further addition of four nucleotide triphosphates,
each labeled on the base with a different fluorescent acceptor dye,
4) excitation of the donor fluorophore with light of a wavelength
specific for the donor but not for any of the acceptors, resulting
in the FRET transfer of the energy associated with the excited
state of the donor to the acceptor fluorophore for a given
nucleotide, which is then radiated, 5) identification of the
nucleotides most recently added to the primer by recording the
fluorescent spectrum of the individual dye molecules at specific
locations in the microscope field, and 6) converting the sequential
spectrum into a DNA sequence for each DNA molecule in the
microscope field of view.
Development
Status: Basic research
IP Status:
- U.S. Patent No. 6,982,146 issued 03 Jan 2006
- U.S. Patent Application No. 11/204,367 filed 12 Aug 2005
Contact
Information:
John D. Hewes, Ph.D., NCI
Technology Transfer Center
Phone: 301-435-3121
E-mail: Hewesj@mail.nih.gov
Reference: #383 JH
Updated 11/02/2007