Testing Information

Testing Status of Agents at NTP

Diisopropylcarbodiimide

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http://ntp.niehs.nih.gov/go/TS-M930063

CASRN: 693-13-0

Formula: C7-H14-N2

Synonyms/Common Names:

  • DIC
  • 1,3-DIISOPROPYLCARBODIIMIDE
  • N,N'-DIISOPROPYLCARBODIIMIDE

Known Uses:

AS A PEPTIDE COUPLING REAGENT; AS A CONDENSING REAGENT IN DIPEPTIDE SYNTHESIS; IN PREPARATION OF POLYIMIDE PRECURSOR COATINGS FOR ELECTROPHORETIC IMAGE DISPLAY FABRICATION; AS A MILITARY NERVE AGENT STABILIZER.

Nomination Background

Short-Term Toxicity

  • 2-WEEK (Topical Application)  (C93020) Completed 
    • NTIS No. PB2007107705  (Peer Review 09/28/2005)
      Citation: Toxicology and Carcinogenesis Studies of Diisopropylcarbodiimide (CAS No. 693-13-0) in F344/N Rats and B6C3F1 Mice (Dermal Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R&M: 0, 10, 30, 90, 269, OR 806 MG/ML; 5/SEX/SPECIES/GROUP
  • 21-WEEK (Topical Application)  (C93020B) Report Complete 
    •   (Peer Review 09/28/2005) Citation: Toxicology Study of Diispropylcarbodiimide (CAS No. 693-13-0) in Genetically Modified (FVB Tg.AC Hemizygous) Mice and Carcinogenicity Study of Diispropylcarbodiimide in Genetically Modified [B6.129-Trp53tm1Brd (N5) Haploinsufficient Mice (Dermal Studies)
    • Mice: Tg.AC (FVB/N) Hemizygous
    • Dose: FEMALE MICE: 0, 4.38, 8.75, 17.5, 35, OR 70 MG/KG; 10/GROUP
  • 27-WEEK (Topical Application)  (C93020C) Report Complete 
    •   (Peer Review 09/28/2005) Citation: Toxicology Study of Diispropylcarbodiimide (CAS No. 693-13-0) in Genetically Modified (FVB Tg.AC Hemizygous) Mice and Carcinogenicity Study of Diispropylcarbodiimide in Genetically Modified [B6.129-Trp53tm1Brd (N5) Haploinsufficient Mice (Dermal Studies)
    • Mice: P53 +/- (C57BL/6)
    • Dose: FEMALE MICE: 0, 4.38, 8.75, 17.5, 35, OR 70 MG/KG; 15/GROUP
  • 13-WEEK (Topical Application)  (C93020) Report Complete 
    • NTIS No. PB2007107705  (Peer Review 09/28/2005)
      Citation: Toxicology and Carcinogenesis Studies of Diisopropylcarbodiimide (CAS No. 693-13-0) in F344/N Rats and B6C3F1 Mice (Dermal Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0, 10, 20, 40, 80, OR 160 MG/KG M: 0, 17.5, 35, 70, 140, OR 280 10 SEX/SPECIES/GROUP, 6 GROUPS

Long-Term Carcinogenicity

  • 2-YEAR (Topical Application)  (C93020D) Report Complete 
    • TR-523 (NTIS No. PB2007107705)  (Peer Review 09/28/2005)
      Toxicology and Carcinogenesis Studies of Diisopropylcarbodiimide (CAS No. 693-13-0) in F344/N Rats and B6C3F1 Mice (Dermal Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: R&M: 0, 10, 20, OR 40 MG/KG; 50/SEX/SPECIES

Special Studies

  • Absorption Disposition Metabolism Elimination (Topical Application; Intravenous)  (S0662) Completed 
    • Citation: "DISPOSITION OF DERMALLY AND IV ADMINISTERED [14C]DIISOPROPYLCARBODIIMIDE IN FISCHER 344 RATS AND B6C3F1 MICE" 1/4/99
    • M Rats: F344/N; M Mice: B6C3F1; M Rats: F344/N
    • Dose: dermal: 4-42 mg/kg (rats); 7-82 mg/kg (mice) IV: 8 mg/kg (dose vol. 1.3 ml/kg) (mice-only) for dermal needed to use charcoal-impregnated cellulose filters

Genetic Toxicology

  • Micronucleus  (A04322) Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Mice: B6C3F1
    • Male Inconclusive 
  • Micronucleus  (A22799) Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Mice: B6C3F1
    • Male Positive 
    • Female Positive 
  • Micronucleus  (A55884) Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Mice: B6C3F1
    • Male Positive 
  • Micronucleus  (A76999) Completed 
    • Citation: Witt, K.L., Tice, R.R., Shelby, M.D., Chhabra, R.S., and Zeiger, E. Induction of Micronucleated Erythrocytes in Rodents by Diisopropylcarbodiimide and Dicyclohexylcarbodiimide: Dependence on Exposure Protocol. Environ. Molec. Mutag. Vol. 33 (1999) 65-74
    • Rats: F344
    • Male Negative 
  • Salmonella  (A69920) Completed 
    •  Inconclusive 
  • Salmonella  (A92772) Completed 
    •  Negative 

Organ Systems Toxicity

  • 4 DAYS Immunotoxicity (Topical Application)  (IMM95010) Completed 
    • F Mice: B6C3F1
    • Dose: .03, .15, .3, 1.5, AND 3% DIC IN ACETONE (99.8%) FOR SENSIZATION, AND 10% DIC FOR CHALLENGE IN THE MEST AND 1.5, 3 AND 10% DIC IN THE LLNA. .25% DNFB USED AS POSITIVE CONTROL FOR SENSITIZATION, AND .5% FOR CHALLENGE.

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Web page last updated on January 22, 2009