Testing Status of Agents at NTP
Methylene blue trihydrate
CASRN: 7220-79-3
Formula: C16 H18 N3 S CL.3H20
Synonyms/Common Names:
- C.I. BASIC BLUE 9, TRIHYDRATE
- PHENOTHIAZIN-5-IUM, 3,7-BIS(DIMETHYLAMINO)-,CHLORIDE, TRIHYDRATE
Known Uses:
BLUE DYE USED IN BACTERIOLOGY, PAPER, SURGICAL SKIN MARKING, ANTIHEMOGLOBINEMIC, CYANINE POISONING ANTIDOTE, FORMERLY USED IN HAIR COLORING
Nomination Background
Short-Term Toxicity
- 13-WEEK (Gavage)
(C91005B) Completed
- Rats: F344/N; Mice: B6C3F1
- Dose: R&M: 0, 25, 50, 100, OR 200 MG/KG; 30 SEX/SPECIES/GROUP
- 4-WEEK (Gavage)
(C91005) Completed
- Rats: F344/N; Mice: B6C3F1
- Dose: R&M: 0, 0.125, 0.25, 0.5, 1.0, OR 2.0 MG/KG BODY WT. R: 20/GROUP/SEX; M: 10/GROUP/SEX
Long-Term Carcinogenicity
- 2-YEAR (Gavage)
(C91005C) Report Review
- TR-540
(Peer Review 06/12/2006)
Toxicology and Carcinogenesis Studiies of Methylene Blue Trihydrate (CAS No. 7220-79-3) in F344/N Rats and B6C3F1 Mice (gavage studies) - Rats: F344/N; Mice: B6C3F1
- Carcinogenesis Results
- Male Rats Some Evidence
- Female Rats No Evidence
- Male Mice Some Evidence
- Female Mice Equivocal Evidence
- Dose: R: 0, 5, 25, OR 50 MG/KG; M: 0, 2.5, 12.5, OR 25 MG/KG; 50/SEX/SPECIES
- TR-540
(Peer Review 06/12/2006)
Special Studies
- Toxicokinetic Study (Intravenous; Gavage; Intravenous; Gavage)
(S0660) Completed
- Citation: PART OF TWO YEAR TECHNICAL REPORT C91005C
- M/F Rats: F344/N; M/F Rats: F344/N; M/F Mice: B6C3F1; M/F Mice: B6C3F1
- Dose: IV: 0.625 mg/ml (rats, 2 ml/kg); 0.125 mg/ml (mice, 4 ml/kg)
Genetic Toxicology
- In Vitro Cytogenetics (CA/SCE)
(563848) Completed
- Chromosome Aberation Positive
- Sister Chromatid Exchange Positive
- Micronucleus
(A76244) Completed
- Mice: B6C3F1
- Male Negative
- Micronucleus
(A77225) Completed
- Mice
- Male Negative
- Female Negative
- Salmonella
(811345) Completed
- Positive
- Salmonella
(A35880) Completed
- Positive
Organ Systems Toxicity
- Conventional Teratology (Gavage)
(TER92124) Completed
- NTIS No. PB93-219574
Citation: Final Report on the Developmental Toxicity of Methylene Blue Trihydrate in Sprague-Dawley (CD) Rats - Rats: Sprague Dawley
- Rats Maternal Positive
- Rats Fetal Positive
- Dose: REP I: 0, 50, 200, 350 MG/KG/DAY REP II: 0, 50, 125, 200 MG/KG/DAY
- NTIS No. PB93-219574
- GD 6 THROUGH 19 Conventional Teratology (Gavage)
(TER92125) Completed
- NTIS No. PB94-207214
Citation: Final Report on the Developmental Toxicity of Methylene Blue Trihydrate in New Zealand White Rabbits - NTIS No. PB94-207503
Citation: Final Report on the Developmental Toxicity of Methylene Blue Trihydrate in New Zealand White Rabbits - F Rabbit: New Zealand White
- Rabbits Maternal Positive
- Rabbits Fetal Positive
- Dose: 0, 50, 100, 150 MG/KG/DAY
- NTIS No. PB94-207214
- GD 6 THROUGH 15 Teratology Pilot Studies (Gavage)
(TRP91018) Completed
- F Rats: Sprague Dawley
- Dose: 0, 50, 100, 200, 400, OR 600 MG/KG
- GD 6 THROUGH 19 Teratology Pilot Studies (Gavage)
(TRP91019) Completed
- F Rabbit: New Zealand White
- Dose: 0, 10, 50, 100, 200, OR 300 MG/KG
Web page last updated on January 22, 2009