National Toxicology Program

CASRN: 96-69-5

Formula: C22 H30 O2 S

Synonyms/Common Names:

• SANTONOX
• TBBC
• 4,4'-THIOBIS(2-(1,1-DIMETHYLETHYL)-5-METHYLPHENOL (9CI)

Known Uses:

ANTIOXIDANT FOR SYNTHETIC & NATURAL RUBBER & LATEXES, STABILIZER FOR POLYETHYLENE FOOD PACKAGING (HSDB 1990).

Short-Term Toxicity

• 14-Day (Dosed-Feed)  (C61610A) Completed 
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Dosed-Feed)  (C61610A) Completed 
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,250,5000, M: 0,500,10000 PPM/5 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C61610B) Report Complete 
  • TR-435 (NTIS No. PB95-225751)  (Peer Review 06/22/1993)
    Toxicology and Carcinogenesis Studies of 4,4'-Thiobis(6-t-butyl-m-cresol) (CAS No. 96-69-5) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats No Evidence 
  • Female Rats No Evidence 
  • Male Mice No Evidence 
  • Female Mice No Evidence 
  • Dose: R: 0,.05,.1,.25, M: 0,.025,.05,.1 %

Special Studies

• Absorption Disposition Metabolism Elimination (Intravenous)  (S0674) Completed 
  • Citation: BORGHOFF, S.J., STEFANKSI, S.A. & BIRNBAUMN, L.S. THE EFFECT OF AGE ON THE GLUCORONIDATION & TOXICITY OF 4,4-THIOBIS(6-TERT-BUTYL-M-CRESOL) TOXICOL & APPL PHARM, 92:453-466. 1988
  • Rats: F344/N
• Absorption Disposition Metabolism Elimination (Topical Application)  (S0675) Completed 
  • Citation: BIRMBAUN, L.S. & HEANEY, S.M., DERMAL ABSORPTION OF THE ANTIOXIDANT 4,4-THIOBIS(6-TERT-BUTYL-M-CRESOL) IN SENCAR MICE & FISCHER RATS, TOXICOL LETTERS, 37:13-19, 1987
  • Mice: Sencar; Rats: F344/N
• Absorption Disposition Metabolism Elimination (Gavage; Intravenous)  (S0677) Completed 
  • Citation: BIRNBAUM, L.S., EASTIN, W.C., JOHNSON, L, & MATTHEWS, H.B.: DISPOSITION OF 4,4-THIOBIS(6-TERT-BUTYL-M-CRESOL) IN RATS, DRUG MET. & DISPOSITION, 11 (6):537-543, 1983
  • Rats: F344/N; Rats: F344/N
• Metabolism (Intravenous)  (S0676) Completed 
  • Citation: SMITH, R.W., MATTHEWS, H.B., PARKER, C.E., & HASS, J.R.: IDENTIFICATION OF THE MAJOR METABOLITE OF 4,4-THIOBIS(6-TERT-BUTYL-M-CRESOL) : BIOMEDICAL MASS SPECTROMETRY, 208-214, VOL 12, #5, 1985
  • M Rats: F344/N

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (395386) Completed 
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Positive 
• Salmonella  (755282) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., Mortelmans, K., and Speck, W. Salmonella mutagenicity tests. III. Results from the testing of 225 chemicals Environ. Mutagen. Vol. 9 (Suppl 9) (1987) 1-109
  •  Negative 

Organ Systems Toxicity

• 14 DAYS Immunotoxicity (Gavage)  (IMM95009) Completed 
  • F Mice: B6C3F1
  • NO OVERT TOXICITY
  • Dose: 0, 10, 100, OR 200 MG/KG
• Neurotoxicology Assessment (Dosed-Feed)  (NTA94011) Completed 
  • Citation: Animal Neurobehavioral Test Battery in NTP Assessment In: Advances in Neurobehavioral Toxicology: Applications in Environmental & Occupational Health (Barry L. Johnson, ED.), Lewis Publishers, Inc. pp. 403-418 (1990).
  • M/F Rats: F344/N
• GD 6-15 (COPULATORY PLUG = GD 0) Short-Term in vivo Repro. Tox. (Gavage)  (T0043) Completed 
  • F Mice: Swiss