National Toxicology Program

CASRN: 2602-46-2

Formula: C32-H24-N6-O14-S4.4NA

Synonyms/Common Names:

• 3,3'-((1,1'-BIPHENYL)-4,4'-DIYLBIS(AZO))BIS(5-AMINO-4-HYDROXY-2,7-NAPHTHALENEDISULFONIC ACID TETRASODIUM SALT (9CI)
• BLUE 6, C.I. DIRECT

Known Uses:

DYE FOR TEXTILES, LEATHER, INK; BIOLOGICAL AND WOOD STAINS; HAIR DYES (FORMERLY)

Short-Term Toxicity

• 2-WEEK (Dosed-Feed)  (C54579A) Completed 
  • NTIS No. PB280204  (Peer Review 03/06/1978)
    Citation: 13-Week Subchronic Toxicity Studies of Direct Blue 6, Direct Black 38, and Direct Brown 95 Dyes
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0, 190, 375, 750, 1500, OR 3000 PPM M: 0, 750, 1500, 3000, 6000, OR 12500 PPM; 10 PER GROUP
• 13-WEEK (Dosed-Feed)  (C54579A) Report Complete 
  • NTIS No. PB280204  (Peer Review 03/06/1978)
    Citation: 13-Week Subchronic Toxicity Studies of Direct Blue 6, Direct Black 38, and Direct Brown 95 Dyes
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0, 750, 1500, 2000, 6000, OR 12500 PPM M: 0, 190, 375, 750, 1500, OR 3000 PPM
• 13-Week (Dosed-Water)  (C54579B) Completed 
  • Rats: F344/N
  • Dose: 0,.019,.3 %/10 PER GROUP

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (160428) Completed 
  • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Negative 
• Mouse Lymphoma  (831122) Completed 
  •  Positive 
• Salmonella  (241000) Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Negative 
• Salmonella  (849964) Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Negative 
• Salmonella Reduction  (461569) Completed 
  • Citation: Reid, T., Morton K., and King, C. Mutagenicity of azo dyes following metabolism by different reductive/oxidative systems. Environ. Mutagen. Vol. 6 (1984) 705-717
  •  Positive 

Organ Systems Toxicity

• GD 6-15 Conventional Teratology (Subcutaneous Injection)  (T0123) Completed 
  • F Mice: Swiss
  • Dose: 0, 50, 75, 100, 200 MG/KG/DAY