Testing Information

Testing Status of Agents at NTP

HC blue 2

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http://ntp.niehs.nih.gov/go/TS-10788-R

CASRN: 33229-34-4

Formula: C12-H19-N3-O5

Synonyms/Common Names:

  • BLUE 2, HC
  • 2,2'-((4-((2-HYDROXYETHYL)AMINO)-3-NITROPHENYL)IMINO)BIS ETHANOL (9CI)

Known Uses:

DYE IN SEMI-PERMANENT HAIR DYES. (TDB)

Short-Term Toxicity

  • 14-Day (Dosed-Feed)  (C54897) Completed 
    • NTIS No. PB86-108339  (Peer Review 07/27/1984)
      Citation: Toxicology and Carcinogenesis Studies of HC Blue No. 2 [2,2'-((4-((2-Hydroxyethyl)amino)-3-nitrophenyl)imino)bis(ethanol)] (CAS No. 33229-34-4) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Dosed-Feed)  (C54897) Completed 
    • NTIS No. PB86-108339  (Peer Review 07/27/1984)
      Citation: Toxicology and Carcinogenesis Studies of HC Blue No. 2 [2,2'-((4-((2-Hydroxyethyl)amino)-3-nitrophenyl)imino)bis(ethanol)] (CAS No. 33229-34-4) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Dose: 0,3100,50000 PPM/10 PER GROUP

Long-Term Carcinogenicity

  • 2-YEAR (Dosed-Feed)  (C54897) Report Complete 
    • TR-293 (NTIS No. PB86-108339)  (Peer Review 07/27/1984)
      Toxicology and Carcinogenesis Studies of HC Blue No. 2 [2,2'-((4-((2-Hydroxyethyl)amino)-3-nitrophenyl)imino)bis(ethanol)] (CAS No. 33229-34-4) in F344/N Rats and B6C3F1 Mice (Feed Studies)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats No Evidence 
      • Female Rats No Evidence 
      • Male Mice No Evidence 
      • Female Mice No Evidence 
    • Dose: MR&MM: 0,5000,10000, FR&FM: 0,10000,20000 PPM/50 PER GROUP

Genetic Toxicology

  • Chromosome Aberrations  (908121) Completed 
    • Mice
    • Chromosome Aberation  Negative 
  • In Vitro Cytogenetics (CA/SCE)  (849045) Completed 
    • Citation: Loveday, K.S., Anderson, B.E., Resnick, M.A., and Zeiger, E. Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro V: Results with 46 chemicals Environ. Molec. Mutagen. Vol. 16 (1990) 272-303
    • Chromosome Aberation Negative 
    • Sister Chromatid Exchange Positive 
  • Drosophila  (519386) Completed 
    • Citation: Foureman, P., Mason, J.M., Valencia, R., Zimmering, S. Chemical Mutagenesis Testing in Drosophila. X. Results of 70 coded compounds tested for the National Toxicology Program. Environ. Molec. Mutagen. Vol. 23 (1994) 208-227
    • Reciprocal Translocation/Sex-Linked Recessive Lethal Negative 
  • Mouse Lymphoma  (880283) Completed 
    • Citation: Myhr, B. and Caspary, W. Chemical mutagenesis at the TK locus in L5178Y mouse lymphoma cells. I. Results for 31 coded compounds in the National Toxicology Program. Environ. Molec. Mutagen. Vol. 18 (1991) 51-83
    •  Positive 
  • Salmonella  (373757) Completed 
    • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelmans, K. Salmonella mutagenicity tests. IV. Results from the testing of 300 chemicals Environ. Molec. Mutagen. Vol. 11 (Suppl 12) (1988) 1-158
    •  Positive 
  • Sister Chromatid Exchanges  (908121) Completed 
    • Mice
    • Sister Chromatid  Positive 

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Web page last updated on January 22, 2009