National Toxicology Program

CASRN: 20325-40-0

Related: 3,3'-DIMETHOXYBENZIDINE 119-90-4

Formula: C14-H16-N2-O2.2CL-H

Synonyms/Common Names:

• 3,3'-DIMETHOXY-(1,1'-BIPHENYL)-4,4'-DIAMINE DIHYDROCHLORIDE (9CI)

Known Uses:

PRODUCTION OF DYES

Short-Term Toxicity

• 14-Day (Dosed-Water)  (C99989) Completed 
  • NTIS No. PB90-241076  (Peer Review 06/27/1989)
    Citation: Toxicology and Carcinogenesis Studies of 3,3'-Dimethoxybenzidine Dihydrochloride (CAS No. 20325-40-0) in F344/N Rats (Drinking Water Studies)
  • Rats: F344/N
• 13-Week (Dosed-Water)  (C99989) Completed 
  • NTIS No. PB90-241076  (Peer Review 06/27/1989)
    Citation: Toxicology and Carcinogenesis Studies of 3,3'-Dimethoxybenzidine Dihydrochloride (CAS No. 20325-40-0) in F344/N Rats (Drinking Water Studies)
  • Rats: F344/N
  • Dose: 0, 170, 330, 630, 1250, 2500 PPM (10 PER GROUP)

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Water)  (C99989) Report Complete 
  • TR-372 (NTIS No. PB90-241076)  (Peer Review 06/27/1989)
    Toxicology and Carcinogenesis Studies of 3,3'-Dimethoxybenzidine Dihydrochloride (CAS No. 20325-40-0) in F344/N Rats (Drinking Water Studies)
  • Rats: F344/N
  • Carcinogenesis Results
  • Male Rats Clear Evidence 
  • Female Rats Clear Evidence 
  • Dose: R: 0,80,170,330 PPM /70,45,75,70 PER GROUP RESPECTIVELY

Special Studies

• Metabolism (Dosed-Feed)  (S0017) Completed 
  • Citation: LYNN, RK, DONIELSON, DW, ILIAS, AM, KENNISH, JM, WONG,K & MATTHEWS, HB: METABOLISM OF BISAZOBIPHENYL DYES DERIVED FROM BENZIDINE, 3,3'-DIMETHYLBENZIDINE OR 3,3'-DIMETHOXYBENZIDINE TO CARCINOGENIC AROMATIC AMINES IN THE DOG & RAT. TOXICOL APPL PHARMACOL, 56:248-258,1980.
  • Rats; Dog

Genetic Toxicology

• Mouse Lymphoma (NCI)  (640872) Completed 
  • Citation: Mitchell, A.D., Rudd, C.J., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for 63 coded chemicals tested at SRI International. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 37-102
  • Mouse Lymphoma-NCI  Positive 
• Mouse Lymphoma (NCI)  (948661) Completed 
  • Citation: Myhr, B.C., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for sixty-three coded chemicals tested at Litton Bionetics, Inc. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 103-194
  • Mouse Lymphoma-NCI  Positive