National Toxicology Program

CASRN: 18662-53-8

Formula: C6 H9 N O6.3NA.H2 O

Synonyms/Common Names:

• GLYCINE, N,N-BIS(CARBOXYMETHYL)-, TRISODIUM SALT, MONOHYDRATE (9CI)
• NITRILOTRIACETIC ACID, TRISODIUM SALT, MONOHYDRATE
• NTA TRISODIUM SALT MONOHYDRATE

Known Uses:

DETERGENTS

Short-Term Toxicity

• 14-Day (Dosed-Feed)  (C01445B) Completed 
  • NTIS No. PB266177  (Peer Review 03/25/1977)
    Citation: Bioassays of Nitrilotriacetic Acid (NTA) and Nitrilotriacetic Acid, Trisodium Salt, Monohydrate (Na3-NTA-H2O) for Possible Carcinogenicity (CAS No. 139-13-9) (NTA) (CAS No. 18662-53-8) (Na3-NTA-H2O)
  • Rats: F344/N; Mice: B6C3F1
• 13-WEEK (Dosed-Feed)  (C01445A) Completed 
  • NTIS No. PB266177  (Peer Review 03/25/1977)
    Citation: Bioassays of Nitrilotriacetic Acid (NTA) and Nitrilotriacetic Acid, Trisodium Salt, Monohydrate (Na3-NTA-H2O) for Possible Carcinogenicity (CAS No. 139-13-9) (NTA) (CAS No. 18662-53-8) (Na3-NTA-H2O)
  • Rats: F344/N
• 13-Week (Dosed-Feed)  (C01445B) Completed 
  • NTIS No. PB266177  (Peer Review 03/25/1977)
    Citation: Bioassays of Nitrilotriacetic Acid (NTA) and Nitrilotriacetic Acid, Trisodium Salt, Monohydrate (Na3-NTA-H2O) for Possible Carcinogenicity (CAS No. 139-13-9) (NTA) (CAS No. 18662-53-8) (Na3-NTA-H2O)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,31600, M: 0,21600 PPM/5 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C01445A) Report Complete 
  • TR-006 (NTIS No. PB266177)  (Peer Review 03/25/1977)
    Bioassays of Nitrilotriacetic Acid (NTA) and Nitrilotriacetic Acid, Trisodium Salt, Monohydrate (Na3-NTA-H2O) for Possible Carcinogenicity (CAS No. 139-13-9) (NTA) (CAS No. 18662-53-8) (Na3-NTA-H2O)
  • Rats: F344/N
  • Carcinogenesis Results
  • Male Rats Positive 
  • Female Rats Positive 
  • Dose: 0,200,2000,20000 PPM/48 PER GROUP
• 2-Year (Dosed-Feed)  (C01445B) Report Complete 
  • TR-006 (NTIS No. PB266177)  (Peer Review 03/25/1977)
    Bioassays of Nitrilotriacetic Acid (NTA) and Nitrilotriacetic Acid, Trisodium Salt, Monohydrate (Na3-NTA-H2O) for Possible Carcinogenicity (CAS No. 139-13-9) (NTA) (CAS No. 18662-53-8) (Na3-NTA-H2O)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Equivocal 
  • Female Rats Equivocal 
  • Male Mice Negative 
  • Female Mice Negative 
  • Dose: R: 0,7500,15000, M: 0,2500,5000 PPM/50 PER GROUP

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (696583) Completed 
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Weakly Positive 
• Mouse Lymphoma (NCI)  (G05650) Completed 
  • Citation: Myhr, B.C., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for sixty-three coded chemicals tested at Litton Bionetics, Inc. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 103-194
  • Mouse Lymphoma-NCI  Negative 
• Mouse Lymphoma (NCI)  (G89169) Completed 
  • Citation: Mitchell, A.D., Rudd, C.J., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for 63 coded chemicals tested at SRI International. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 37-102
  • Mouse Lymphoma-NCI  Negative 
• Salmonella (NCI)  (N-9211) Completed 
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli Environ. Mutagen. Vol. 7 (Suppl 5) (1985) 1-248
  • Salmonella-NCI  Negative 
• Salmonella  (051920) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelman, K. Salmonella Mutagenicity Tests V. Results from the Testing of 311 Chemicals Environ. Molec. Mutagen. Vol. 19 (Suppl 21) (1992) 2-141
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
  •  Negative