National Toxicology Program

CASRN: 6373-74-6

Formula: C18-H14-N4-O7-S.NA

Synonyms/Common Names:

• 5-((2,4-DINITROPHENYL)AMINO)-2-(PHENYLAMINO)-BENZENESULFONIC ACID MONOSODIUM SALT (9CI)
• ORANGE 3, C.I. ACID

Known Uses:

DYEING ACETATE, WOOL, NYLON, SILK, AND LEATHER; SHADING COMPONENT IN SOAPS AND WOOD STAINS. A DINITRODIPHENYLAMINE DERIVATIVE USED IN SEMIPERMANENT HAIR COLORING PRODUCTS.

Short-Term Toxicity

• 14-Day (Gavage)  (C54911) Completed 
  • NTIS No. PB89-216550  (Peer Review 07/14/1987)
    Citation: Toxicology and Carcinogenesis Studies of C.I. Acid Orange 3 (CAS No. 6373-74-6) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Gavage)  (C54911) Completed 
  • NTIS No. PB89-216550  (Peer Review 07/14/1987)
    Citation: Toxicology and Carcinogenesis Studies of C.I. Acid Orange 3 (CAS No. 6373-74-6) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,94,1500, M: 0,250,2000 MG/KG/10 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Gavage)  (C54911) Report Complete 
  • TR-335 (NTIS No. PB89-216550)  (Peer Review 07/14/1987)
    Toxicology and Carcinogenesis Studies of C.I. Acid Orange 3 (CAS No. 6373-74-6) in F344/N Rats and B6C3F1 Mice (Gavage Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats No Evidence 
  • Female Rats Clear Evidence 
  • Male Mice No Evidence 
  • Female Mice No Evidence 
  • Dose: R: 0,375,750 MG/KG MM: 0,125,250 MG/KG FM: 0,250,500 MG/KG/50 PER GROUP

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (835697) Completed 
  • Chromosome Aberation Positive 
  • Sister Chromatid Exchange Positive 
• Salmonella  (537306) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., and Mortelmans, K. Salmonella mutagenicity tests. IV. Results from the testing of 300 chemicals Environ. Molec. Mutagen. Vol. 11 (Suppl 12) (1988) 1-158
  •  Positive