National Toxicology Program

CASRN: 121-14-2

Formula: C7-H6-N2-O4

Synonyms/Common Names:

• 1-METHYL-2,4-DINITROBENZENE (9CI)

Known Uses:

DYE INTERMEDIATE; EXPLOSIVES

Short-Term Toxicity

• 14-Day (Dosed-Feed)  (C01865) Completed 
  • NTIS No. PB280990  (Peer Review 01/18/1978)
    Citation: Bioassay of 2,4-Dinitrotoluene for Possible Carcinogenicity (CAS No. 121-14-2)
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Dosed-Feed)  (C01865) Completed 
  • NTIS No. PB280990  (Peer Review 01/18/1978)
    Citation: Bioassay of 2,4-Dinitrotoluene for Possible Carcinogenicity (CAS No. 121-14-2)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: 0,.00375,.015 %/10 PER GROUP

Long-Term Carcinogenicity

• 2-Year (Dosed-Feed)  (C01865) Report Complete 
  • TR-054 (NTIS No. PB280990)  (Peer Review 01/18/1978)
    Bioassay of 2,4-Dinitrotoluene for Possible Carcinogenicity (CAS No. 121-14-2)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Positive 
  • Female Rats Positive 
  • Male Mice Negative 
  • Female Mice Negative 
  • Dose: R: 0,.008,.02 (AVG), M: 0,.008,.04 (AVG) %/50 PER GROUP

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (322897) Completed 
  • Citation: Loveday, K.S., Lugo, M.H., Resnick, M.A., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro II: Results with 20 chemicals Environ. Molec. Mutagen. Vol. 13 (1989) 60-94
  • Chromosome Aberation Negative 
  • Sister Chromatid Exchange Positive 
• Drosophila  (050763) Completed 
  • Citation: Woodruff, R.C., Mason, J.M., Valencia, R., and Zimmering, S. Chemical mutagenesis testing in Drosophila: V. Results of 53 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 677-702
  • Sex-Linked Recessive Lethal Positive 
  • Reciprocal Translocation Negative 
• Drosophila  (957177) Completed 
  • Citation: Woodruff, R.C., Mason, J.M., Valencia, R., and Zimmering, S. Chemical mutagenesis testing in Drosophila: V. Results of 53 coded compounds tested for the National Toxicology Program. Environ. Mutagen. Vol. 7 (1985) 677-702
  • Reciprocal Translocation/Sex-Linked Recessive Lethal Inconclusive 
• Mouse Lymphoma  (693724) Completed 
  •  Positive 
• Salmonella (NCI)  (N-9340) Completed 
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1984) Reproducibility of microbial mutagenicity assays. I. Tests with Salmonella typhimurium and Escherichia coli using a standardized protocol. Environ. Mutagen. 6 (Suppl. 2): 1-251.
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. Reproducibility of microbial mutagenicity assays. I. Tests with Salmonella typhimurium and Escerichia coli using a standardized protocol Environ. Mutagen. Vol. 6 (Suppl 2) (1985) 1-251
  • Salmonella-NCI  Positive 
• Salmonella  (683014) Completed 
  • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
  • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1984) Reproducibility of microbial mutagenicity assays. I. Tests with Salmonella typhimurium and Escherichia coli using a standardized protocol. Environ. Mutagen. 6 (Suppl. 2): 1-251.
  •  Positive 

Organ Systems Toxicity

• 8 CONS D (MTD PHASE), GD 7-14 (REPRO PHASE) Short-Term in vivo Repro. Tox. (Gavage)  (T0090) Completed 
  • NTIS No. PB84-183540
    Citation: Determination of the Reproductive Effects in Mice of Nine Selected Chemicals
  • F Mice: Swiss
  • Dose: 390 MG/KG BODY WT.