Testing Information

Testing Status of Agents at NTP

4-Nitro-o-phenylenediamine

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http://ntp.niehs.nih.gov/go/TS-10476-J

CASRN: 99-56-9

Formula: C6-H7-N3-O2

Synonyms/Common Names:

  • 4-NITRO-1,2-BENZENEDIAMINE (9CI)

Known Uses:

HAIR DYE; REAGENT FOR ALPHA-KETOACIDS, ASCORBIC AND DEHYDROASCORBIC ACIDS

Short-Term Toxicity

  • 14-Day (Dosed-Feed)  (C03941) Completed 
    • NTIS No. PB290306  (Peer Review 10/25/1978)
      Citation: Bioassay of 4-Nitro-o-phenylenediamine for Possible Carcinogenicity (CAS No. 99-56-9)
    • Rats: F344/N; Mice: B6C3F1
  • 13-Week (Dosed-Feed)  (C03941) Completed 
    • NTIS No. PB290306  (Peer Review 10/25/1978)
      Citation: Bioassay of 4-Nitro-o-phenylenediamine for Possible Carcinogenicity (CAS No. 99-56-9)
    • Rats: F344/N; Mice: B6C3F1
    • Dose: R: 0,681,10000, M: 0,1470,21500 PPM/5 PER GROUP

Long-Term Carcinogenicity

  • 2-Year (Dosed-Feed)  (C03941) Report Complete 
    • TR-180 (NTIS No. PB290306)  (Peer Review 10/25/1978)
      Bioassay of 4-Nitro-o-phenylenediamine for Possible Carcinogenicity (CAS No. 99-56-9)
    • Rats: F344/N; Mice: B6C3F1
    • Carcinogenesis Results
      • Male Rats Negative 
      • Female Rats Negative 
      • Male Mice Negative 
      • Female Mice Negative 
    • Dose: R: 0,375,750, M: 0,3750,7500 PPM/50 PER GROUP

Genetic Toxicology

  • Chromosome Aberrations  (704945) Completed 
    • Mice
    • Chromosome Aberation  Negative (Nonstandard Protocol) 
  • In Vitro Cytogenetics (CA/SCE)  (549927) Completed 
    • Chromosome Aberation Negative 
    • Sister Chromatid Exchange Positive 
  • Mouse Lymphoma (NCI)  (G19468) Completed 
    • Citation: Mitchell, A.D., Rudd, C.J., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for 63 coded chemicals tested at SRI International. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 37-102
    • Mouse Lymphoma-NCI  Positive 
  • Mouse Lymphoma (NCI)  (G31547) Completed 
    • Citation: Myhr, B.C., and Caspary, W.J. Evaluation of the L5178Y mouse lymphoma cell mutagenesis assay: Intralaboratory results for sixty-three coded chemicals tested at Litton Bionetics, Inc. Environ. Molec. Mutagen. Vol. 12 (Suppl 13) (1988) 103-194
    • Mouse Lymphoma-NCI  Positive 
  • Micronucleus  (A82327) Completed 
    • Mice: B6C3F1
    • Male Inconclusive 
  • Salmonella (NCI)  (N-9239) Completed 
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli Environ. Mutagen. Vol. 7 (Suppl 5) (1985) 1-248
    • Salmonella-NCI  Positive 
  • Salmonella  (274391) Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
    •  Positive 
  • Salmonella  (541901) Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
    •  Positive 
  • Salmonella  (803155) Completed 
    • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
    • Citation: Dunkel, V., Zeiger, E., Brusick, D., McCoy, E., McGregor, D., Mortelmans, K., Rosenkranz, H., and Simmon, V. (1985) Reproducibility of microbial mutagenicity assays. II. Testing of carcinogens and noncarcinogens in Salmonella typhimurium and Escerichia coli. Environ. Mutagen. 7 (Suppl 5): 1-248.
    •  Positive 
  • Sister Chromatid Exchanges  (704945) Completed 
    • Mice
    • Sister Chromatid  Negative (Nonstandard Protocol) 

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Web page last updated on January 22, 2009