National Toxicology Program

CASRN: 95-80-7

Related: TRANSGENIC MODEL EVALUATION (2,4-DIAMINOTOLUENE)

Formula: C7-H10-N2

Synonyms/Common Names:

• 4-METHYL-1,3-BENZENEDIAMINE (9CI)
• 2,4-TOLUENE DIAMINE

Known Uses:

INTERMEDIATE FOR POLYURETHANES AND DYES; IN HAIR DYES (FORMERLY)

Short-Term Toxicity

• 13-WEEK (Dosed-Feed)  (C02302) Completed 
  • NTIS No. PB293593  (Peer Review 12/13/1978)
    Citation: Bioassay of 2,4-Diaminotoluene for Possible Carcinogenicity (CAS No. 95-80-7)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,250,3000, M: 0,100,1000 PPM/5 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C02302) Report Complete 
  • TR-162 (NTIS No. PB293593)  (Peer Review 12/13/1978)
    Bioassay of 2,4-Diaminotoluene for Possible Carcinogenicity (CAS No. 95-80-7)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Positive 
  • Female Rats Positive 
  • Male Mice Negative 
  • Female Mice Positive 
  • Dose: MR: 0,79,176 (AVG), FR: 0,79,171 (AVG), M: 0,100,200 PPM/50 PER GROUP

Special Studies

• Cell Proliferation (Gavage)  (S0696) Completed 
  • Citation: CUNNINGHAM, M.L., FOLEY,J., MARONPOT, R.R., MATTHEWS, H.B. CORRELATION OF HEPATOCELLULAR PROLIFERATION WITH HEPATOCARCINOGENICITY INDUCED BY THE MUTAGENIC NONCARCINOGEN:CARCINOGEN PAIR - 2,6- & 2,4-DIAMINOTOLUENE TOXICOLOGY & APPLIED PHARM 107, 562-567 (1991)
  • M Rats: F344/N
• Mechanisms (Intraperitoneal Injection)  (S0697) Completed 
  • Citation: CUNNINGHAM, M.L., & MATTHEWS, H.B. EVIDENCE FOR AN ACETOXYARYLAMINE AS THE ULTIMATE MUTAGENIC REACTIVE INTERMEDIATE OF THE CARCINOGENIC AROMATIC AMINE 2,4-DIAMINOTOLUENE, MUTATION RESEARCH, 242 (1990) 101-110
  • M Rats: F344/N
• Mechanisms  (S0698) Completed 
  • Citation: CUNNINGHAM, M.L., BURKA, L.T., MATTHEWS, H.B., THE INTERACTION OF METHANOL, RAT-LIVERS S9 AND THE AROMATIC AMINE 2,4-DIAMINOTOLUENE PRODUCES A NEW MUTAGENIC COMPOUND: MUTATION RESEARCH, 244 (1990) 273-277
  • M Rats: F344/N
• Metabolism  (S0699) Completed 
  • Citation: CUNNINGHAM, M.L., MATTHEWS, H.B., BURKA, L.T.: ACTIVATION OF METHANOL BY HEPATIC POSTMITOCHONDRIAL SUPERNATANT: FORMATION OF A CONDENSATION PROUDCT WITH 2,4-DIAMINOTOLUENE. CHEMICAL RESEARCH TOXICOLOGY, VOL3, #2 157-161, 1990.
  • M Rats: F344/N
• Metabolism  (S0700) Completed 
  • Citation: FURLONG, B.B., WEAVER, R.P., & GOLDSTEIN, J.A. COVALENT BINDING TO DNA & MUTAGENICITY OF 2,4-DIAMINOTOLUENE METABOLITES PRODUCED BY ISOLATERD HEPATOCYTES AND 9000 G SUPERNATANT FROM FISCHER 344 RATS, CARCINOGENSIS VOL 8, #2 247-251, 1987
  • M Rats: F344/N
• Other (Intraperitoneal Injection)  (S0701) Completed 
  • Citation: HAYWARD, J.J., SHANE, B.S. TINDALL, K.R., & CUNNINGHAM, M.L.: DIFFERENTIAL IN VIVO MUTAGENICITY OF THE CARCINOGEN/NON-CARCINGEN PAIR 2,4- & 2,6-DIAMINOTOLUENE: CARCINOGENESIS VOL 16 NO 10, PP 2429-2433, 1995
  • M Mice: B6C3F1

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (314882) Completed 
  • Citation: Loveday, K.S., Anderson, B.E., Resnick, M.A., and Zeiger, E. Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro V: Results with 46 chemicals Environ. Molec. Mutagen. Vol. 16 (1990) 272-303
  • Chromosome Aberation Positive 
  • Sister Chromatid Exchange Positive 
• Micronucleus  (520516) Completed 
  • Mice: B6C3F1
  • Male Negative 
• Micronucleus  (571690) Completed 
  • Mice: B6C3F1
  • Male Negative 
  • Female Negative 
• Salmonella  (707834) Completed 
  • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
  •  Positive 

Organ Systems Toxicity

• 14 DAY Immunotoxicity (Gavage)  (IMM87034) Completed 
  • F Mice: B6C3F1
  • Dose: 0, 25, 50, 100 MG/KG
• Immunotoxicity (Topical Application)  (IMM92040) Completed 
  • F Mice: B6C3F1
  • Dose: 0, 1, 3, 10%
• 8 CONS D (MTD PHASE), GD 7-14 (REPRO PHASE) Short-Term in vivo Repro. Tox. (Gavage)  (T0039) Completed 
  • NTIS No. PB84-183540
    Citation: Determination of the Reproductive Effects in Mice of Nine Selected Chemicals
  • F Mice: Swiss
  • Dose: 150 MG/KG BODY WT.