National Toxicology Program

CASRN: 91-23-6

Formula: C7-H7-N-O3

Synonyms/Common Names:

• 1-METHOXY-2-NITROBENZENE (9CI)
• NITROPHENYL METHYL ETHER

Known Uses:

USED AS AN INTERMEDIATE FOR THE PREPARATION OF O-ANISIDINE AND IN THE MANUFACTURE OF AZO DYES

Short-Term Toxicity

• 14-Day (Dosed-Feed)  (C60388A) Completed 
  • NTIS No. PB94-109758
    Citation: Toxicology and Carcinogenesis Studies of o-Nitroanisole (CAS No. 91-23-6) in F344 Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
• 13-Week (Dosed-Feed)  (C60388A) Completed 
  • NTIS No. PB94-109758
    Citation: Toxicology and Carcinogenesis Studies of o-Nitroanisole (CAS No. 91-23-6) in F344 Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: R: 0,200,18000, M: 0,60,6000 PPM/10 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C60388B) Report Complete 
  • TR-416 (NTIS No. PB94-109758)  (Peer Review 11/21/1991)
    Toxicology and Carcinogenesis Studies of o-Nitroanisole (CAS No. 91-23-6) in F344 Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1; Rats: F344/N
  • Carcinogenesis Results
  • Male Rats Clear Evidence 
  • Female Rats Clear Evidence 
  • Male Mice Clear Evidence 
  • Female Mice Some Evidence 
  • Dose: (CORE STUDY) R: 0, 222, 666, 2000 PPM M: 0, 666, 2000, 6000 PPM (STOP STUDY) R: 0, 6000, 18000 PPM; 50/GROUP

Special Studies

• Absorption Disposition Metabolism Elimination (Intravenous; Gavage)  (S0028) Completed 
  • Citation: MILLER, M.J., SIPES, I.G., PERRY, D.F. & CARTER, D.E.: PHARMACOKINETICS OF O-NITROANISOLE IN F344 RATS, DRUG MET. & DISP., 13(5):527-531, 1985
  • M Rats: F344/N; M Rats: F344/N
  • Dose: PO: 5,50 MG/KG IV: 25 MG/KG

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (410303) Completed 
  • Citation: Galloway, S., Armstrong, M., Reuben, C., Colman, S., Brown, B., Cannon, C., Bloom, A., Nakamura, F., Ahmed, M., Duk, S., Rimpo, J., Margolin, B., Resnick, M., Anderson, B., and Zeiger, E. Chromosome aberration and sister chromatid exchanges in Chinese hamster ovary cells: Evaluations of 108 chemicals. Environ. Molec. Mutagen. Vol. 10 (Suppl 10) (1987) 1-176
  • Chromosome Aberation Weakly Positive 
  • Sister Chromatid Exchange Positive 
• Mouse Lymphoma  (578918) Completed 
  •  Positive 
• Salmonella  (178197) Completed 
  •  Positive 
• Salmonella  (205865) Completed 
  • Citation: Haworth, S., Lawlor, T., Mortelmans, K., Speck, W., and Zeiger, E. Salmonella mutagenicity test results for 250 chemicals Environ. Mutagen. Vol. 5 (Suppl 1) (1983) 3-142
  •  Positive