National Toxicology Program

CASRN: 77-09-8

Formula: C20-H14-O4

Synonyms/Common Names:

• 3,3-BIS(4-HYDROXYPHENYL)-1(3H)-ISOBENZOFURANONE (9CI)

Known Uses:

LABORATORY REAGENT. DYES. CATHARTIC DRUG IN LAXATIVES. (TDB)

Short-Term Toxicity

• 13-WEEK (Dosed-Feed)  (C55798) Completed 
  • NTIS No. PB97-169882
    Citation: Toxicology and Carcinogenesis Studies of Phenolphthalein (CAS No. 77-09-8) in F344 Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: 0,.3,5 %/5 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C55798B) Report Complete 
  • TR-465 (NTIS No. PB97-169882)  (Peer Review 12/05/1995)
    Toxicology and Carcinogenesis Studies of Phenolphthalein (CAS No. 77-09-8) in F344 Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Clear Evidence 
  • Female Rats Some Evidence 
  • Male Mice Clear Evidence 
  • Female Mice Clear Evidence 
  • Dose: R: 0, 1.2, 2.5, OR 5%; M: 0, 0.3, 0.6, OR 1.2% IN FEED (50/SEX/SPECIES/GROUP)

Special Studies

• Absorption Disposition Metabolism Elimination (Gavage)  (S0755) Completed 
  • Citation: GRIFFIN, R.J., GODFREY, V.B., AND BURKA, L.T. METABOLISM AND DISPOSITION OF PHENOLPHTHALEIN IN MALE AND FEMALE F344 RATD AND B6C3F1 MICE. TOXICOLOGICAL SCIENCES 42, 73-81 (1998)
  • M/F Rats: F344/N; M/F Mice: B6C3F1
  • Dose: 800 mg/kg
• Toxicokinetic Study (Dosed-Feed; Intravenous)  (S0594) Completed 
  • M/F Rats: F344/N; M/F Mice: B6C3F1; M/F Rats: F344/N; M/F Mice: B6C3F1
  • Dose: Feed: Mice: 200, 375, 750, 3000, and 12000 ppm Feed: Rats: 375, 750, 3000, 12000, and 50000 ppm iv: 25 mg/kg for both species and sexes

Genetic Toxicology

• Chromosome Aberrations  (A53649) Completed 
  • Mice
  • Chromosome Aberation  Negative (Nonstandard Protocol) 
• In Vitro Cytogenetics (CA/SCE)  (101813) Completed 
  • Chromosome Aberation Positive 
  • Sister Chromatid Exchange Negative 
• Micronucleus  (A13109) Completed 
  • Mice: P16(INK4A)/(+/-) (C57BL/6)
  • Male Positive 
  • Female Positive 
• Micronucleus  (A20259) Completed 
  • Mice: P16(INK4A)/(+/-) (C57BL/6)
  • Male Positive 
  • Female Positive 
• Micronucleus  (A34828) Completed 
  • Mice: P16(INK4A)/(+/-) (C57BL/6)
  • Male Positive 
  • Female Positive 
• Micronucleus  (A93351) Completed 
  • Citation: Witt, K.L., Knapton, A., Wehr, C.M., Hook, G.J., Mirsalis, J., Shelby, M.D., and MacGregor, J.T. Micronucleated Erythrocyte Frequency in Peripheral Blood of B6C3F1 Mice from Short-Term, Prechronic, and Chronic Studies of the NTP Carcinogenesis Bioassay Program. Environ. Molec. Mutagen. Vol. 36 (2000) 163-194
  • Mice: B6C3F1
  • Male Positive 
  • Female Positive 
• Micronucleus  (A93723) Completed 
  • Mice: P16(INK4A)/(+/-) (C57BL/6)
  • Male Positive 
  • Female Positive 
• Micronucleus  (A93901) Completed 
  • Mice: B6C3F1
  • Male Positive 
• Salmonella  (041772) Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Negative 
• Salmonella  (768193) Completed 
  • Citation: Mortelmans, K., Haworth, S., Lawlor, T., Speck, W., Tainer, B., and Zeiger, E. Salmonella mutagenicity tests. II. Results from the testing of 270 chemicals Environ. Mutagen. Vol. 8 (Suppl 7) (1986) 1-119
  •  Negative 
• Salmonella  (A71314) Completed 
  •  Inconclusive 
• Salmonella  (A99430) Completed 
  •  Negative 

Organ Systems Toxicity

• >120 DAYS Continuous Breeding (Dosed-Feed)  (RACB89066) Completed 
  • NTIS No. PB91-178707
    Citation: Final Report on the Reproductive Toxicology of Phenolphthalein in CD-1 Swiss Mice
  • M/F Mice: Swiss
  • Dose: N/A; 0,.1,.7,3%; 0,.7%; 0,.1,.7%