National Toxicology Program

CASRN: 59-87-0

Formula: C6-H6-N4-O4

Synonyms/Common Names:

• 2-((5-NITRO-2-FURANYL)METHYLENE)HYDRAZINECARBOXAMIDE (9CI)

Known Uses:

ANTIBACTERIAL AGENT, SENSITIZER AND FOOD ADDITIVE, EXPERIMENTAL CARCINOGEN

Short-Term Toxicity

• 14-Day (Dosed-Feed)  (C56064) Completed 
  • NTIS No. PB89-102388  (Peer Review 07/14/1987)
    Citation: Toxicology and Carcinogenesis Studies of Nitrofurazone (CAS No. 59-87-0) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
• 7-WEEK (Dosed-Feed)  (C95005) Completed 
  • Mice: B6C3F1
  • Dose: FEMALE MICE ONLY: 0 OR 310 PPM
• 13-Week (Dosed-Feed)  (C56064) Completed 
  • NTIS No. PB89-102388  (Peer Review 07/14/1987)
    Citation: Toxicology and Carcinogenesis Studies of Nitrofurazone (CAS No. 59-87-0) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Dose: 20 PER GROUP

Long-Term Carcinogenicity

• 2-YEAR (Dosed-Feed)  (C56064) Report Complete 
  • TR-337 (NTIS No. PB89-102388)  (Peer Review 07/14/1987)
    Toxicology and Carcinogenesis Studies of Nitrofurazone (CAS No. 59-87-0) in F344/N Rats and B6C3F1 Mice (Feed Studies)
  • Rats: F344/N; Mice: B6C3F1
  • Carcinogenesis Results
  • Male Rats Equivocal Evidence 
  • Female Rats Clear Evidence 
  • Male Mice No Evidence 
  • Female Mice Clear Evidence 
  • Dose: R: 0,310,620, M: 0,150,310 PPM/50 PER GROUP
• 2-YEAR (Dosed-Feed)  (C95005) Completed 
  • Mice: B6C3F1
  • Dose: FEMALE MICE ONLY: 0 OR 310 PPM

Genetic Toxicology

• In Vitro Cytogenetics (CA/SCE)  (356548) Completed 
  • Citation: Anderson, B.E., Zeiger, E., Shelby, M.D., Resnick, M.A., Gulati, D.K., Ivett, J.L., and Loveday, K.S. Chromosome aberration and sister chromatid exchange test results with 42 chemicals. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 55-137
  • Chromosome Aberation Positive 
  • Sister Chromatid Exchange Positive 
• Mouse Lymphoma  (267199) Completed 
  • Citation: Myhr B.C., McGregor, D., Bowers, L., Riach, C., Brown, A., Edwards, I., McBride, D., Martin, R., and Caspary, W.J. L5178Y mouse lymphoma cell mutation assay results with 41 compounds. Environ. Molec. Mutagen. Vol. 16 (Suppl 18) (1990) 138-167
  •  Positive 
• Micronucleus  (A59017) Completed 
  • Mice: B6C3F1
  • Male Negative 
• Salmonella  (364720) Completed 
  • Citation: Zeiger, E., Anderson, B., Haworth, S., Lawlor, T., Mortelmans, K., and Speck, W. Salmonella mutagenicity tests. III. Results from the testing of 225 chemicals Environ. Mutagen. Vol. 9 (Suppl 9) (1987) 1-109
  •  Positive 

Organ Systems Toxicity

• Immunotoxicity (Gavage)  (IMM90011) Completed 
  • NTIS No. PB92-140383
    Citation: Summary Reports on Immunotoxicology Vol. 1, No. 1-15
  • Citation: SUMMARY (1-15)
  • F Mice: B6C3F1
  • Mice Female Negative 
  • Dose: 0, 40, 60, 80 MG/KG
• 27 WEEKS Continuous Breeding (Dosed-Feed)  (RACB89020) Completed 
  • NTIS No. PB92-217694
    Citation: Final Report on the Reproductive Toxicity of Nitrofurazone in CD-1 Swiss Mice
  • M/F Mice: Swiss
  • Dose: 0,100,200,400,600,900 PPM; 0,100,375,750 PPM; 0,750 PPM; 0,100,375 PPM
• 8 CONS D (MTD PHASE), GD 7-14 (REPRO PHASE) Short-Term in vivo Repro. Tox. (Gavage)  (T0012A) Completed 
  • NTIS No. PB83-258616
    Citation: Screening of Priority Chemicals for Potential Reproductive Hazard
  • F Mice: Swiss
  • Dose: 100 MG/KG BODY WT
• GD 6-15 Conventional Teratology (Dosed-Feed)  (TER85092) Completed 
  • NTIS No. PB86-145844
    Citation: Teratologic Evaluation of Nitrofurazone Administered to CD-1 Mice on Gestational Days 6 Through 15
  • F Mice: Swiss
  • Mice Maternal Positive 
  • Mice Fetal Positive 
  • Dose: 0, 0.0038, 0.0075, 0.025, 0.050%
• GD 6-19 Conventional Teratology (Gavage)  (TER87093) Completed 
  • NTIS No. PB88-130984
    Citation: Teratologic Evaluation of Nitrofurazone Administered to New Zealand White Rabbits on Gestational Days 6-19
  • F Rabbit: New Zealand White
  • Rabbits Maternal Positive 
  • Rabbits Fetal Positive 
  • Dose: 0, 5, 10, 15, 20 MG/KG/DAY