Background:
The HIV Drug Resistance Program,
National Cancer Institute at Frederick is seeking statements of
capability or interest from parties interested in collaborative
research to further develop, evaluate, or commercialize Research
Tools.
Technology:
The invention describes a
DNA-binding molecule that allows recovery of viral DNA and
associated proteins. An acridine orange based molecule was modified
and the resulting alkylating acridine molecule intercalates with
viral pre-integration complexes (PIC) or other DNAs localized in
cytosol. Because the molecule is also biotinylated, streptavidin
beads can be used to purify the molecule and the bound DNA and
associated protein can subsequently be eluted and analyzed. The
invention provides a useful tool to facilitate the studies for
viral PIC and other cytosol DNAs. It is available for non-exclusive
licensing as biological material and research
tool.
R&D Status:
Pre-clinical, in vitro data available
Contact
Information:
John D. Hewes, Ph.D., NCI
Technology Transfer Center
Phone: 301-435-3121
E-mail: Hewesj@mail.nih.gov
Reference: #448 KH