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High-Speed Parallel Molecular Nucleic Acid Sequencing

Background:
The NCI Nanobiology Program is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize nanoscale or molecular nucleic acid sequencing. The IP is also available for co-exclusive licensing.

Technology:
The technology is a new system, methods and compositions for DNA sequencing, also known as Two Dye Sequencing (TDS). This invention is based on Fluorescence Resonance Energy Transfer (FRET), a technology increasingly in use for several molecular analysis purposes. In particular, the method consists of: 1) attachment of engineered DNA polymerases labeled with a donor fluorophore to the surface (chamber) of a microscope field of view, 2) addition to the chamber of DNA with an annealed oligonucleotide primer and template, which is bound by the polymerase, 3) further addition of four nucleotide triphosphates, each labeled on the base with a different fluorescent acceptor dye, 4) excitation of the donor fluorophore with light of a wavelength specific for the donor but not for any of the acceptors, resulting in the FRET transfer of the energy associated with the excited state of the donor to the acceptor fluorophore for a given nucleotide, which is then radiated, 5) identification of the nucleotides most recently added to the primer by recording the fluorescent spectrum of the individual dye molecules at specific locations in the microscope field, and 6) converting the sequential spectrum into a DNA sequence for each DNA molecule in the microscope field of view.

Development Status: Basic research

IP Status:
  • U.S. Patent No. 6,982,146 issued 03 Jan 2006
  • U.S. Patent Application No. 11/204,367 filed 12 Aug 2005

Contact Information:
John D. Hewes, Ph.D., NCI Technology Transfer Center
Phone: 301-435-3121
E-mail: Hewesj@mail.nih.gov

Reference:  #383 JH

Updated 11/02/2007

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Page Last Updated: 12-17-2008