The National Institute of Diabetes & Digestive & Kidney Diseases

Molecular Mechanisms of DNA Repair

The lab’s focus is on molecular and cellular studies of DNA mismatch repair proteins including their roles in post-replication repair, DNA damage signaling, activation of cell cycle and checkpoint controls , and the roles of mismatch repair proteins in homologous recombination and meiosis. Approaches include biochemistry, structural biology, cell biology, and molecular genetics.

1. Yoshioka K, Yoshioka Y, Hsieh P  ATR kinase activation mediated by MutSalpha and MutLalpha in response to cytotoxic O6-methylguanine adducts.  Mol Cell (22): 501-10, 2006. [Full Text/Abstract]

2. Yang Y, Sass LE, Du C, Hsieh P, Erie DA  Determination of protein-DNA binding constants and specificities from statistical analyses of single molecules: MutS-DNA interactions.  Nucleic Acids Res (33): 4322-34, 2005. [Full Text/Abstract]

3. Wang H Yang Y Schofield MJ Du C Fridman Y Lee SD Larson ED Drummond JT Alani E Hsieh P Erie DA  DNA bending and unbending by MutS govern mismatch recognition and specificity.  Proc Natl Acad Sci U S A (100): 14822-7, 2003. [Full Text/Abstract]

4. Schofield MJ Hsieh P  DNA mismatch repair: molecular mechanisms and biological function.  Annu Rev Microbiol (57): 579-608, 2003. [Full Text/Abstract]

5. Selmane T Schofield MJ Nayak S Du C Hsieh P  Formation of a DNA mismatch repair complex mediated by ATP.  J Mol Biol (334): 949-65, 2003. [Full Text/Abstract]

6. Junop MS Obmolova G Rausch K Hsieh P Yang W  Composite active site of an ABC ATPase: MutS uses ATP to verify mismatch recognition and authorize DNA repair.  Mol Cell (7): 1-12, 2001. [Full Text/Abstract]

7. Schofield MJ Nayak S Scott TH Du C Hsieh P  Interaction of Escherichia coli MutS and MutL at a DNA mismatch.  J Biol Chem (276): 28291-9, 2001. [Full Text/Abstract]

8. Obmolova G Ban C Hsieh P Yang W  Crystal structures of mismatch repair protein MutS and its complex with a substrate DNA.  Nature (407): 703-10, 2000. [Full Text/Abstract]