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Abstract
Grant Number: 5F05AT002014-04 Project Title: Molecular Basis of Berberine-Mediated Antitumor Activity
PI Information: Name Title KANG, QUAN kp1028@yahoo.com Abstract: DESCRIPTION (provided by applicant): The objective of this fellowship application is to determine the gene expression profile in berberine-treated human cancer cells. An increasing amount of attention has been paid to the use of complementary and alternative medicine (CAM) as a part of the treatment for human cancer. A Chinese herb, Coptis chinensis, that is routinely used in China for treating gastroenteritis for thousands of years, may be developed as an anti-tumor agent. Coptis contains multiple constituents, and berberine is the major constituent. We have recently demonstrated that Coptis extract and berberine exhibit significant anti-proliferative activity in human cancer cells. However, the molecular mechanisms underlying Coptis or berberine-mediated anti-tumor activity are unknown. In this application, I propose to conduct a comprehensive gene expression profiling analysis in berberine-treated human cancer cells by focusing on three specific aims. The first aim is to conduct hybridizations of RNA samples prepared from berberine-treated human cancer cells. The second aim is to conduct comprehensive bioinformatic analyses on the microarray data set. The third aim is to verify potentially important targets of berberine using Northern blotting or quantitative RT-PCR. Taken together, by analyzing the expression profile of nearly 40,000 genes, I expect to identify a set of target genes that may function as intracellular mediators of berberine's anti-cancer action. My long-term goal is to elucidate the molecular bases of berberine-mediated antitumor activity, and to investigate the possible use of berberine and its derivatives as effective chemotherapy and/or chemopreventive agents in human cancer.
Public Health Relevance:
This Public Health Relevance is not available.Thesaurus Terms:
alkaloid, antineoplastic, drug discovery /isolation, gene expression, gene expression profiling, plant extract
functional /structural genomics, molecular biology information system
human genetic material tag, microarray technology, neoplastic cell, northern blotting, nucleic acid hybridization, polymerase chain reaction, postdoctoral investigator
Institution: CHONGQING UNIVERSITY OF MEDICAL SCIENCES #136, ZHONG-SAN 2 RD CHONGQING, Fiscal Year: 2007 Department: Project Start: 01-DEC-2003 Project End: 30-NOV-2007 ICD: NATIONAL CENTER FOR COMPLEMENTARY & ALTERNATIVE MEDICINE IRG: ZAT1