Dicentric Chromosomes
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Scoring of Dicentrics
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![closeup of slide showing a dicentric spread of 45 chromosomes](RemmMockup_files/cbl-step9b-2.jpg) |
Image A | Image B |
- A digital photo of the metaphase spread is taken at 63x magnification.
- A dicentric chromosome has two centromeres — the point where the two chromatids connect.
- The number of dicentric chromosomes is counted and compared to calibration curves to estimate radiation dose.
- Image A displays a normal metaphase spread.
- Image B reveals a meatphase spread with a dicentric chromosome circled in red.
Adapted from Cytogenetic Biodosimetry Laboratory: The Process (Radiation Emergency Assistance Center/Training Site (REAC/TS))
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